食品科学 ›› 2026, Vol. 47 ›› Issue (12): 141-152.doi: 10.7506/spkx1002-6630-20251117-125

• 生物工程 • 上一篇    

酱香型白酒第2~4轮次发酵中微生物群落演替的理化驱动机制

文方龄,赵毓,李生锋,游接华,梁立杰,姚闽娜,林永辉   

  1. (1.福建农林大学食品科学学院,福建 福州 350002;2.福建省农业科学院农业质量标准与检测技术研究所,福建 福州 350003;3.福建双龙戏珠酒业有限公司,福建 南平 354200)
  • 发布日期:2026-07-08
  • 基金资助:
    福建省公益类竞争性项目(2023R1064);福建省“5511”协同创新工程项目(XTCXGC2021020); 福建省自然科学基金项目(2025J011227)

Physicochemical Mechanism Driving Microbial Community Succession in the 2nd to 4th Rounds of Jiangxiangxing Baijiu Fermentation

WEN Fangling, ZHAO Yu, LI Shengfeng, YOU Jiehua, LIANG Lijie, YAO Minna, LIN Yonghui   

  1. (1. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2. Institute of Quality Standards & Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou 350003, China; 3. Fujian Shuanglong Xizhu wine industry Co., Ltd., Nanping 354200, China)
  • Published:2026-07-08

摘要: 本研究针对酱香型白酒第2~4轮次这一发酵阶段,通过监测酒醅上、中、底层理化指标并解析微生物群落,系统揭示其微生态互作规律。结果表明,发酵过程呈现规律性动态变化:水分含量与酸度持续上升,淀粉被持续消耗,乙醇体积分数呈“抛物线”型变化。这一理化动态揭示了微生物活动驱动下的淀粉消耗、乙醇生成及酸类积累的核心转化过程。微生物层面,细菌群落(以Acetilactobacillus为核心)高度稳定,而真菌群落的核心菌属发生显著演替(从Schizosaccharomyces转变到Pichia),且核心酵母菌表现出明显的“亲上层”分布特征。冗余分析与相关性分析进一步阐明,驱动群落演替的核心环境因子随轮次发生序贯更替:初期(第2轮次)由水分和淀粉含量主导初始定植;转折期(第3轮次)乙醇成为塑造细菌群落的主导胁迫因子;至后期(第4轮次)则形成由酸度主导、真菌在乙醇合成中作用凸显的稳定体系。本研究从微生态角度揭示了第2~4轮次发酵中理化环境与微生物群落时空互作的动态规律,可为传统酿造工艺的精准调控与品质管理提供科学依据。

关键词: 酱香型白酒;微生物群落结构;理化指标;动态变化特征

Abstract: This study investigated the physicochemical indicators and microbial communities in the top, middle and bottom layers of fermented grains of Jiangxiangxing Baijiu in the 2nd to 4th rounds of fermentation, and it also systematically revealed the pattern of their microecological interactions. The results showed that the fermentation process exhibited regular dynamics: moisture and acidity continued to rise, starch was continuously consumed, and alcohol content showed a parabola-like trend. These physicochemical dynamic changes revealed the core transformation process of starch consumption, ethanol production and acid accumulation driven by microbial activities. The bacterial community (with Acetilactobacillus as the core) was highly stable, while the core fungal genera underwent significant succession (from Schizosaccharomyces to Pichia), and the core yeasts were mainly distributed in the upper layer. Redundancy analysis and correlation analysis further clarified that the core environmental factors driving the community succession sequentially shifted with the rounds: water and starch contents dominated microbial colonization at the initial stage (the 2nd round). During the transition period (the third round), alcohol content became the dominant stress factor shaping the bacterial community. In the later stage (the fourth round), a stable system dominated by acidity featuring the role of fungi in alcohol synthesis was formed. By revealing the dynamic pattern of the spatiotemporal interaction between the physicochemical environment and microbial communities during the 2nd to 4th rounds of fermentation from a microecological perspective, this study provides a scientific basis for the precise regulation and quality management of the traditional brewing process.

Key words: Jiangxiangxing Baijiu; microbial community structure; physicochemical indicators; dynamic change characteristics

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