食品科学 ›› 1998, Vol. 19 ›› Issue (5): 10-13.

• 食品科学 • 上一篇    下一篇

乳链球菌乳糖利用阴性突变体的分离及其性质研究

 冉雪琴, 王嘉福, 朱秋劲, 叶在荣   

  1. 贵州农学院
  • 出版日期:1998-05-15 发布日期:2011-12-12

 RAN  Xue-Qin, WANG  Jia-Fu, ZHU  Qiu-Jin, YE  Zai-Rong   

  • Online:1998-05-15 Published:2011-12-12

摘要: 以紫外线和溴乙锭对乳链球菌6030和6025菌株诱变,筛选出乳糖代谢障碍(Lac-)突变体,测定了乳链球菌及其突变体利用糖类的能力和β-半乳糖苷酶活力,经质粒DNA检测和Southcrn印迹杂交分析,证实乳链球菌Lac-突变体乳糖代谢能力的丧失与含有乳糖代谢基因的大质粒有关。

关键词: 乳链球菌, Lac-突变体, &beta, -半乳精苷酶

Abstract: Inctose-negative mutants were derived from streptococcus lactis 6030 and 6025 treated by ultravi-olet irradiation and ethidium bromide. The nature of the carbohydrates fermention and the β- galactase activity were determined in the mutants. The β-galactase genes of S. Lactis 6030, 6025 and Lacmutants were analyzed by a DIG-β-galactase gene probe.The results showed that the lactose metabolism of S·Lactis was associated with the presence of the plasmid.

Key words: Streptococcus lactis; Lac-mutant; &beta, -galactase;