食品科学 ›› 2000, Vol. 21 ›› Issue (4): 13-19.

• 基础研究 • 上一篇    下一篇

以谷物淀粉为碳源的培养基中葡萄糖的动态变化

吴文惠,安藤功一   

  1. 内蒙古农业大学食品科学与工程, 日本酪农学园大学乳制品制造研究室
  • 出版日期:2000-04-15 发布日期:2011-11-29

Glucose Variation in Cultural Medium Taking Cereal Starch as Carbon Source

 WU  Wen-Hui, AN  Teng-Gong-Yi   

  • Online:2000-04-15 Published:2011-11-29

摘要: 以小麦淀粉、玉米淀粉、马铃薯淀粉和可溶性淀粉为精源代替葡萄糖为碳源的MRS培养基上检测了Lactobacil-Lus amylovorus JCM 1126和Lactobacillus amylophilus JCM 1125的葡萄糖代谢特征同时测定了pH值、活菌数的变化和增殖状况。 Lactobacilius amylovorus JCM 1126从淀粉分解出的葡萄糖迅速的被用于菌体的繁殖生长,活菌数的增殖表现出单峰曲线, pH的降低同葡萄糖做碳源的培养基几乎是一致的。与此相对Lactobdcillus amylophilus JCM 1125从淀粉分解出的葡萄糖当累积到较多的数量之后菌体的繁殖生长才进入旺盛生长阶段,生长曲线表现出典型的两种糖源存在时的双峰曲线增长现象, pH降低的速度明显慢于葡萄糖做碳源的培养基。两个菌株经长时间的乳酸发酵(48h)后,淀粉做碳源的情况下保持有较葡萄糖做碳源更多的活菌数。 从培养基pH值降低、菌体生长曲线和葡萄糖代谢上分析, Lactobacillus amylovorus JCM 1126和Lactobacil-lus amylophilus JCM 1125对淀粉的分解程度和利用方式是不

关键词: 乳酸菌, 葡萄糖, 活菌数pH, 谷物淀粉

Abstract: Glucose metaboliccharacteristics of Lactobacillus amylovorus JCM1126 and Lactobacillus amylophilns JCM1125I in the MRS cultural medium with two cereal starches and some soluble starch substitute for the glucose were studied. For Lactobacillus amylovorus JCM1126, the graph of colony quantity proliferaton had a single peak, and the drop of pH was similar to that in cultural medium taking glucose as carbon source. For Lactobacillus amylophilus JCM1125, its graph had two peaks, and pH dropping rate was remarkably slower than that taking glucose as carbon source. 48 hours later both bactewla showed more colony quantities taking starches as carbon source than those taking glucose as carbon scurce. As shown above the two bacterias were sharply different in the way they metabolized.

Key words: Lactic acid bacteria Glucose Colony quantity Potential hydrogen Cereal starch