食品科学 ›› 2001, Vol. 22 ›› Issue (1): 23-26.

• 基础研究 • 上一篇    下一篇

银杏淀粉的分离和纯化

 敖自华, 王璋, 许时婴   

  1. 无锡轻工大学食品学院
  • 出版日期:2001-01-15 发布日期:2012-02-14

Study on Extraction and Purification of Amylose and Amylopectin from Ginkgo (Ginkgo Biloba L.) Starch

 AO  Zi-Hua, WANG  Zhang, XU  Shi-Ying   

  • Online:2001-01-15 Published:2012-02-14

摘要: 用重结晶法可以得到纯度较高的银杏直链淀粉和支链淀粉。凝胶过滤色谱表明:银杏直链淀粉的分子量比玉米直链淀粉的小,而支链淀粉的分子量则具有较宽的分布。银杏直、支链淀粉的碘亲和力分别为19.19%和0.13%,蓝值分别是0.85和0.12,λmax为626nm和564nm;银杏淀粉中直链淀粉含量为33%。

关键词: 银杏淀粉, 重结晶, 分离, 纯化, 凝胶过滤色谱

Abstract: Highly purified amylopectin and amylose from ginkgo biloba L.starch could be obtained by recrystallization.Results of gel permeation chromatography (GPC) on sepharose 2B showed that molecular weight of ginkgo amylose appeared to be smaller than that of corn amylose,whereas the amylopectin molecular weight had a wide distribution in the GPC profile.For ginkgo amylose and amylopectin,iodine affinity values were 19.19% and 0.13%,blue values 0.85 and 0.12,and λmax of I2-KI blue colour 626nm and 564nm ,respectively.Amylose content of ginkgo starch was 33%.

Key words: Ginkgo starch Recrystallization Purification Separation GPC