关键词: 高效液相色谱法, 饮料, 添加剂

Abstract: To study the optimum conditions for simultaneous determination of additives in drink, the reversed phase high performance liquid chromatography and the optimum determination wavelength were applied. The Benzoic Acid , Sorbic Acid , Saccharin Sodium , Tartrazine , Amaranth , Ponceau 4R , Sunset Yellow , Brilliant Blue,Caffeien in drink were assayed on C18 reversed-phase column using methanol-0.02mol/L as the mobil phase. The ammonium acetate solution was: methanol: 5% , holding 4min;5%~35%,10%/min;35%~70%,17.5%min at a flow rate of 1ml/min and detected by DAD at 0~5min , 230nm ; 5~7.5min , 400nm ; 7.5~9.25min , 520nm ; 9.25~9.9min , 285nm ; 9.9~11min , 630nm . Good separation was achieved in the 9 components and the standard curve showing a direct ratio between the concentration and the peak area was obtained ,as r>0.999 , CV<1.6%(n=6). The detection limits were below 0.3ng . Recovery percent was found between 93.8% and 109.2% .Both the method and GB method as were used to determine 15 samples respectively. For comparison , the results did not show appreciable difference (P>0.05). The method proved to be satisfactory in precision , sccuracy and sensitivity . The preparation of sample was simple and the work efficiency was high.

Key words: HPLC Drink Additives