食品科学 ›› 2001, Vol. 22 ›› Issue (10): 85-87.
• 分析检测 • 上一篇 下一篇
袁道强, 舒友琴
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YUAN Dao-Qiang, SHU You-Qin
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摘要: 用溶液凝胶法固定α-淀粉酶、葡萄糖淀粉酶和葡萄糖氧化酶,将其用于流动注射化学发光分析体系,可实现对淀粉的快速检测,测定的线性范围为10-200mg/L,检出限为0.2mg/L,10次测定的相对标准偏差为1.8%。
关键词: 溶胶凝胶技术, 固定化酶, 化学发光, 淀粉
Abstract: In this paper,α-amylase,glucoamylase and glucose oxidase were Immobilized by sol-gel.With its application to jlowing injection chemilumine-scence system,starch could be rapidly determined with the liner rage of its concentration in 10-200mg/L and detection limit at 0.2mg/L.The relativte standard deviation (RSD) is 1.8% with 10 continuous assays.
Key words: Sol-gel technique Immobilized Enzyme Chemiluminescence Starch
袁道强, 舒友琴. 固定化酶流动注射化学发光法测定淀粉的含量[J]. 食品科学, 2001, 22(10): 85-87.
YUAN Dao-Qiang, SHU You-Qin. The Determination of Starch by Immobilized Enzyme and Flowing Injection Chemiluminescence[J]. FOOD SCIENCE, 2001, 22(10): 85-87.
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