食品科学 ›› 2001, Vol. 22 ›› Issue (5): 46-48.

• 基础研究 • 上一篇    下一篇

三波长紫外分光光度法测定大豆异黄酮含量的研究

 鞠兴荣, 袁建, 汪海峰   

  1. 南京经济学院食品科学与工程系;
  • 出版日期:2001-05-15 发布日期:2012-02-16

Study on Determination of Soybean Isoflavones Content bythree-wavelength,UV Spectrophotometry

 JU  Xing-Rong, YUAN  Jian, WANG  Hai-Feng   

  • Online:2001-05-15 Published:2012-02-16

摘要: 采用三波长紫外分光光度法测定大豆异黄酮含量,有效地消除了随浓度不同发生的本底漂移、油脂等干扰物质及吸收峰不对称给定量分析造成的影响。方法简便、灵敏,最低检出浓度为1μg/ml,加样回收率>99%,具有良好的准确性和精密度。

关键词: 大豆异黄酮; 定量测定; 三波长&mdash, 紫外分光光度法;

Abstract: Determination of soybean isoflavone content by three-wavelengths UV spectrophotometry was studied to eliminate the absorbance error of interfering components(such as oil).the problen of peak floating which varied changed with concentration change and the asymmetric absorption peak problems could be solved also.The method was more advantageous than traditional spectrophotometer method.The detection limit was 1μg/ml.The average recovery rate was 99.1%with RSD less than 3%.

Key words: Soybean isoflavones Determination.Three-wavelength UV spectrophotometry.