关键词: 菌球, 菌体干重, 总糖, &beta, -CD

Abstract: In this paper,we studied the liquid submerged fermentation process conditions for Tremella facifamis Berk As5.526.Through one-factor experiments,we determined that 0.5% concentration of β-CD thicker is added to liquid medium;and through othogonal experiments L9(33),we determined the optimal formations of oxygen-supplying for shaker:oscillation frequency is 200r/min,500ml flask contatins 150ml meedium,five layers of tela are used.The peloton should be plenty and small,and at high thalline concentration.Thus,the content of dry weight of thalline amounts to 0.5g/100ml,and total sugar to 5.5mg/ml.Moreover,through many experiments,subcultivation period is 36 hours,inoculum concentration is 5%,fermentation temperature is from 24℃ to 26℃,the value of pH is from 6 to 7,fermentation cycle is 72 hours.That is considered as optimum conditions for Tremella facifamis Berk As5.526.

Key words: Peloton , Dry weight of thalline , Total sugar , β-CD