食品科学 ›› 2003, Vol. 24 ›› Issue (11): 118-120.

• 分析检测 • 上一篇    下一篇

桑叶多糖的含量测定

 欧阳臻, 李永辉, 宿树兰, 陈钧   

  1. 江苏大学生物与环境工程学院
  • 出版日期:2003-11-15 发布日期:2011-12-13

Determination of Polysaccharide in Leaves of Morus alba L.

 OU Yang-Zhen , LI  Yong-Hui, SU  Shu-Lan, CHEN  Jun   

  1. College of Biological and Environmental Engineering, Jiangsu University
  • Online:2003-11-15 Published:2011-12-13

摘要: 用蒽酮-硫酸比色法测定了桑叶中多糖的含量。测定波长620nm,多糖换算因子f=3.07;在10~60μg/ml范围内吸收度与被测物含量呈良好的线性关系,r=0.999005;多糖的平均回收率为98.81%,RSD为2.08%(n=5)。测定结果表明,10月份桑叶中多糖含量最高。本法简便、准确、重现性好,可作为桑叶中多糖的含量测定方法。

关键词: 桑叶, 多糖, 硫酸-蒽酮法, 含量测定

Abstract: The contents of polysaccharide in leaves of Morus alba L. were measured by anthrone-H2SO4 colorimetry.Wavelength for measurement was 620nm and converting factor of polysaccharide was 3.07;Good linearity ranged from 10~60μg/ml,r=0.999005;Average recovery rate of polysaccharide was 98.81% with 2.08% of RSD(n=5). The results showedthe highest content of polysaccharide was in leaves of Morus alba L. collected at Oct.; The method used in this paper was simple,accurate and stable. It can be used for determination of the polysaccharide in leaves of Morus alba L..

Key words: leaves of Morus alba L., polysaccharide, anthrone-sulfuric acid determination