食品科学 ›› 2003, Vol. 24 ›› Issue (7): 34-40.

• 基础研究 • 上一篇    下一篇

枸杞子阿拉伯半乳糖聚糖糖蛋白的微细构造研究(I)

 秦小明, 宁恩创, 林华娟   

  1. 广西大学生物技术与糖业工程学院,日本歧阜大学联合农学研究科歧阜市
  • 出版日期:2003-07-15 发布日期:2011-12-13

 QIN  Xiao-Ming, NING  En-Chuang, LIN  Hua-Juan   

  • Online:2003-07-15 Published:2011-12-13

摘要: 对从枸杞子水溶性粗多糖中分离出来的一种主要阿拉伯半乳糖聚糖糖蛋白Cp-2-B的微细构造进行了研究.通过高碘酸氧化和缓和Smith分解,从分解产物中分离出一个分子量为1.8万的半乳糖聚糖 (Cp-2-B-SD)。 甲基化分析和GC-MS分析结果表明Cp-2-B-SD为典型的梳状构造:它的主链是由(1→3)结合的半乳糖残基组成,其中约30%的半乳糖残基带有取代基。另外,还通过蛋白质水解酶反应以及阿拉伯半乳糖聚糖糖蛋白(AGP)与β-galactosylYariv试剂的特有免疫反应等手段,探讨了Cp-2-B的微细构造特征。

关键词: 枸杞子, 阿拉伯半乳糖聚糖糖蛋白(AGP), 微细构造

Abstract: To characterize the fine structure of the predominant arabinogalactan-protein (Cp-2-B) isolated from the fruit ofLycium chinense Mill, a periodate oxidation and Smith degradation were preformed, and a galactan (Cp-2-B-SD, Mw=18,000)was obtained. The result of methylation and GC-MS analysis suggested that Cp-2-B-SD had a typical comb-like structure: abackbone of (1→3) -galactosyl residues was in existence and ca. 30% of the galactosyl residues in the backbone had substituents.Protease enzymatic reaction and an immune reaction with the β- galactosyl Yariv reagent were then examined to characterize thecommon structure of Cp-2-B.

Key words: Lycium chinense Mill, arabinogalactan-protein, fine structure