食品科学 ›› 2004, Vol. 25 ›› Issue (10): 29-32.

• 基础研究 •    下一篇

Alcalase AF 2.4L酶解玉米蛋白动力学研究

 陈新, 陈庆森, 庞广昌   

  1. 天津市食品生物技术重点实验室天津商学院生物工程系
  • 出版日期:2004-10-15 发布日期:2011-10-24

Hydrolysis Dynamics Study on Corn Gluten Meal by Alcalase AF 2.4L

 CHEN  Xin, CHEN  Qing-Sen, PANG  Guang-Chang   

  1. The Tianjin Key Laboratory of Food Biotechnology, Tianjin University of Commerce
  • Online:2004-10-15 Published:2011-10-24

摘要: 本文对碱性蛋白酶Alcalase AF 2.4L水解玉米蛋白的动力学特征进行了较为系统的研究:(1)探讨固液比、酶量、温度、pH值等因素对玉米蛋白水解度的影响,研究确定了碱性蛋白酶Alcalase AF 2.4L水解玉米蛋白的最佳工艺控制条件;(2)根据所建立的pH-stat酶解体系确定了Vmax和Km,为更有效地利用玉米蛋白资源奠定了理论基础。

关键词: 玉米蛋白, AlcalaseAF2.4L, 酶解, 动力学

Abstract: This paper has probed the dynamics of hydrolysis on CGM by Alcalase AF 2.4L: (1) The factors including the ratioof solid to liquid ,adding amount of enzyme,temperature of reaction ,pH value and time of reaction affecting the degree ofhydrolysis of CGM by Alcalase AF 2.4L were studied through single-factor-analysis; (2) The optimal conditions, the maximumvelocity and instant Km were determined through probing into the relation of the ratio of solid to liquid and enzyme reactionvelocity.

Key words: corn gluten mea(CGM), Alcalase AF 2.4L, enzymolysis, dynamics