食品科学 ›› 2004, Vol. 25 ›› Issue (11): 173-176.

• 工艺技术 • 上一篇    下一篇

乳球菌FML02-8合成胞外多糖的条件优化

 陈晓红, 董明盛, 周剑忠, 刘小莉   

  1. 南京农业大学食品科技学院食品微生物研究室
  • 出版日期:2005-11-15 发布日期:2011-10-24

Optimization of Exopolysaccharide Production by Lactococcus FML02-8

 CHEN  Xiao-Hong, DONG  Ming-Sheng, ZHOU  Jian-Zhong, LIU  Xiao-Li   

  1. Lab of Food Microbe, College of Food Science and Technology, Nanjing Agricultural University
  • Online:2005-11-15 Published:2011-10-24

摘要: 用SAS8.0的四因素二水平析因设计筛选了影响EPS合成的显著因素为温度、初始pH和时间,用中心组合设计得出了优化的EPS产量模型并进行了验证。在优化条件下,EPS产量达400mg/L。3L发酵罐的pH控制(流加碱)发酵表明:发酵过程的pH控制5.5时EPS的合成量最高,达503.3mg/L,较未控制时的产量提高了约30%。

关键词: 乳球菌, 胞外多糖, 响应曲面, 条件优化

Abstract: The optimum condttion for the synthesis of EPS from FML02-8 was selected as follows: By means of 24 factorialdesign in SAS8.0 software, remarkable factors affecting the production of EPS were selected as temperature. pH and time.Through a central composite design, a preferable model of the production of EPS was established and tested. The constant pHin 3L fermentation indicated that different constant pH values during fermentation affect the yield of EPS from FML02-8. Underthe constant pH5.5, the yield of EPS was 503.3mg/L, increasing 30% compared without control of pH.

Key words: lactococcus, exopolysaccharide(EPS), response surface design, condition optimization