食品科学 ›› 2004, Vol. 25 ›› Issue (4): 150-152.

• 分析检测 • 上一篇    下一篇

高效液相色谱法测定南果梨中绿原酸的含量

 辛广, 刘长江, 侯冬岩, 吴俊青   

  1. 鞍山师范学院化学系,沈阳农业大学食品学院
  • 出版日期:2004-04-15 发布日期:2011-10-24

Determination of Chlorogenic Acid of Nanguoli Pear by HPLC

 XIN  Guang, LIU  Chang-Jiang, HOU  Dong-Yan, WU  Jun-Qing   

  1. 1.Department of Chemistry,Anshan Normal University;2.College of Food Science, Shenyang Agricultural
  • Online:2004-04-15 Published:2011-10-24

摘要: 目的建立测定南果梨中绿原酸含量的高效液相色谱法。方法C18色谱柱(250×4.6mm,5μm);流动相甲醇-0.4%磷酸溶液(2575);检测波长327nm。结果精密度试验RSD为1.9%,n=6;加样回收率98.5%~102.6%,RSD为1.6%~2.1%。结论南果梨果心、果肉、果皮中绿原酸的含量分别为1.785、0.271、0.714mg/g。

关键词: 高效液相色谱法, 南果梨, 绿原酸

Abstract: Objective: To establish a new HPLC method for determination of chlorogenic acid(CGA)in Nanguoli pear.Method:CGA was analysed by using C18 column(250mm×4.6mm,10μm) with detector at 327nm, and the mobile phase consistingof CH3OH-0.4% H3PO4 (25:75).Results: The recoveries of CGA was 98.5%~102.6%,precision (RSD) was 1.6%~2.1% (n=5).Conclusion:Quantitative analysis showed that the content of CGA in the core peel and pulp of Nanguoli pearwere 1.785, 0.271 and 0.714mg/g respectively.

Key words: HPLC, Nanguoli pear, chlorogenic acid