食品科学 ›› 2004, Vol. 25 ›› Issue (8): 188-190.

• 专题论述 • 上一篇    下一篇

基因芯片技术在食品微生物检测和研究中的应用

 郑大明, 张静   

  1. 山东省疾病预防控制中心
  • 出版日期:2004-08-15 发布日期:2011-10-24

Review on Gene Chip Technology Applications on Food Microbiology Assay

 ZHENG  Da-Ming, ZHANG  Jing   

  1. Shandong Province Centre of Preventive Medicine
  • Online:2004-08-15 Published:2011-10-24

摘要: 基因芯片技术为全面快速准确地分析鉴定水体、空气、土壤和食品等环境中的各种微生物提供了一种崭新的技术工具和平台。本文扼要综述了近年来基因芯片技术在食品微生物检测中的研究进展,着重讨论基因芯片检测微生物的基本原理与步骤, 样品的采集制备和分离纯化食品微生物DNA的方法和要求,基因芯片技术检测食品常见致病菌及其在食品微生物研究中的应用,该技术在食品微生物中的应用现状和前景。

关键词: 基因芯片, 食品微生物, 检测

Abstract: Gene chip technology has provided a new tool and platform for rapid detection of various microbes in multipleenvironments as water,air,soil and food. It was briefly reviewed about recent progresses on testing of food microbes by genechip based procedures. The discussions were focused on the basic principles and steps of testing of food microbes by genechips,collection and preparation of food samples,the methods and requirements for separating and purifying food microbialDNA,detecting and studying of common pathological bacteria in food with gene chip based technology,and the currentstatus and perspectives on the applications of gene chip on food microbiology assay.

Key words: gene chip, food microbe, detection