香菇中5’-核苷酸的高效液相色谱-质谱分析

食品科学 ›› 2005, Vol. 26 ›› Issue (1): 196-198.

香菇中5’-核苷酸的高效液相色谱-质谱分析

邹耀洪

常熟理工学院化学系

出版日期:2005-01-15 发布日期:2011-09-19

Analysis of 5’-Nucleotide in Lentinus Edodes with High Performance Liquid Chromatography-Mass Spectrometry

ZOU Yao-Hong

Department of Chemistry,Changshu Institute of Technology

Online:2005-01-15 Published:2011-09-19

摘要/Abstract

摘要： 采用高效液相色谱/质谱法,对香菇中5’-核苷酸进行了分析。在ODS柱上,以含0.05%磷酸的水-甲醇(90/10,V/V)为流动相进行了分离。以HPLC与LC/MS联合定性香菇中5’-核苷酸为5’-肌苷酸,5’-鸟苷酸,5’-尿苷酸和5’-腺苷酸。采用外标法定量,它们的标准加入回收率分别为100.4%、99.0%、97.8%、和97.2%,相对标准偏差分别为2.07%、2.18%、1.91%和2.76%,检出限分别为2.5、2.1、3.2、和3.5μg/ml。该方法准确可靠,简便易行。

关键词: 高效液相色谱-质谱, 5&rsquo, -核苷酸, 香菇

Abstract: A new high performance liquid chromatography/mass spectrometric method for the analysis of 5’-nucleotide in lentinus edodes was reported. 5’-nucleotide could be separated on a ODS column by using a mobile phase containing 0.05% phosphoric acid water-methanol (90/10,V/V). The 5’-nucleotide in lentinus edodes were identified as 5’-inosine monophosphate, 5’-guanosine monophosphate, 5’-uridine monophosphate and 5’-adenosine monophosphate by using HPLC and LC/MS. The content of four 5’-nucleotides in lentinus edodes was determined by external standard method. Their recoveries were respectively 100.4%, 99.0%, 97.8%, 97.2%, their relative standard deviation were respectively 2.07%, 2.18%, 1.91%, 2.76% and their detection were respectively 2.5, 2.1, 3.2, 3.5μg/ml. The method was accurate, simple and easy.

Key words: high performance liquid chromatography/mass spectrometry, 5’-nucleotide, lentinus edodes

邹耀洪. 香菇中5’-核苷酸的高效液相色谱-质谱分析[J]. 食品科学, 2005, 26(1): 196-198.

ZOU Yao-Hong. Analysis of 5’-Nucleotide in Lentinus Edodes with High Performance Liquid Chromatography-Mass Spectrometry[J]. FOOD SCIENCE, 2005, 26(1): 196-198.

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