食品科学 ›› 2005, Vol. 26 ›› Issue (2): 130-134.

• 工艺技术 • 上一篇    下一篇

长根菇液体深层发酵条件的研究

 邹祥, 胡昌华   

  1. 西南师范大学生命科学学院
  • 出版日期:2005-02-15 发布日期:2011-09-19

Studies on Submerged Fermentation Conditions of Oudemanciella radicata

 ZOU  Xiang, HU  Chang-Hua   

  1. School of Life Science, Southwest China Normal University
  • Online:2005-02-15 Published:2011-09-19

摘要: 研究了各种营养因子对长根菇深层发酵的影响,确定了合适的碳源、氮源、C/N比、无机盐、生长因子的浓度,在初始pH5.5、250ml摇瓶装液量50ml、接种量10%、温度26℃的培养条件下,长根菇深层发酵结果最佳,在此基础上进行摇瓶发酵曲线测定,确定了长根菇适宜发酵周期为96h,发酵液胞外多糖最高可达2.85g/L。

关键词:  , 长根菇, 深层发酵, 胞外多糖

Abstract: The nutrient factors of Oudemanciella radicata were studied in the submerged fermentation. It showed that the suitable of single factors were chosen as: carbon sourse, nitrogen source, C/N ratio, growth factors and inorganic salts. The optimum conditions of fermentations were as follows: initial pH5.5, temperature 26℃, the cultivations performed in 250ml shake flask containing 50ml medium and inoculated to a concentration of 10%. Under these conditions, the time course of growth in shake flask was determined. The results showed that the maximum extracellular polysaccharide(EPS) concentration was 2.85g/L, when the mycelium had been cultured for the optimum cycle of 96h in the shake flask.

Key words: Oudemanciella radicata, submerged fermentation, extracellular polysaccharide