食品科学 ›› 2005, Vol. 26 ›› Issue (8): 465-468.

• 专题论述 • 上一篇    下一篇

PCR-DGGE指纹技术在食品微生物研究中的应用

 高蕙文, 吕欣, 董明盛   

  1. 南京农业大学食品微生物实验室
  • 出版日期:2005-08-15 发布日期:2011-09-19

Application of PCR-DGGE Fingerprinting in Food Microbiology

 GAO  Hui-Wen, LU Xin , DONG  Ming-Sheng   

  1. Food Microbiology Laboratory, Nanjing Agricultural University
  • Online:2005-08-15 Published:2011-09-19

摘要: 分子生物学的发展带动了实验技术的进步,DGGE作为一种不依赖于培养的分子技术最近被用于食品微生物的研究。本文简要介绍了DGGE基本原理,及其在微生物鉴定,种群多样性研究和发酵过程动态监测中的应用,并对该技术自身存在的缺陷进行了评价。

关键词: PCR-DGGE, 食品微生物学, 微生物多样性

Abstract: The development of Molecule Biology brings along the improving of experimental tecnology. DGGE is used in the study of food microbiology as a non-culture method. The principle in this paper is summarized, and the application in several fields of food microbiology are reviewed: the identification of microorganisms, the evaluation of microbial diversity, and mornitoring during food fermentation. Limitations of this approach are also indicated

Key words: PCR-DGGE, food microbiology, microbial diversity