食品科学 ›› 2005, Vol. 26 ›› Issue (9): 29-33.

• 基础研究 •    下一篇

鲜切芦蒿过氧化物酶特性的研究

 郁志芳, 陆兆新, 李妍, 宋雯雯   

  1. 南京农业大学食品科技学院
  • 出版日期:2005-09-15 发布日期:2011-09-20

The Characteristics of the POD from Fresh-cut Artemisia selengensis

 YU  Zhi-Fang, LU  Zhao-Xin, LI  Yan, SONG  Wen-Wen   

  1. College of Food Science and Technology, Nanjing Agricultural University
  • Online:2005-09-15 Published:2011-09-20

摘要: 芦蒿去除不可食部分、冰水清洗、切分后在磷酸缓冲液中用研钵碾成匀浆状于4℃低温下以10000r/min离心30min,获得的过氧化物酶(POD)粗酶液用于酶活力和酶特性的研究。结果表明,鲜切芦蒿中过氧化物酶活力最适宜的pH为5.0,最适宜的温度为40.0℃,且在60℃以上的温度下易失活。H2O2浓度在0.4%以下时,鲜切芦蒿POD活力随浓度增加直线上升,0.6%时达到饱和。鲜切芦蒿POD与不同酚类底物的亲和力顺序为焦性没食子酸>绿原酸>愈创木酚>儿茶酚>酪氨酸>苯酚、间苯二酚。

关键词: 芦蒿, 鲜切, 过氧化物酶(POD), 特性

Abstract: The fresh Artemisia selengensis after leaf and inedible part being removed, was cleaned in ice water and cut and smarshed and centrifuged (10000r/min,30min) at 4℃. The obtained crude POD was used to measure the enzyme activity and investigate the characteristics of the enzyme. The results showed that the POD in the fresh-cut Artemisia selengensis had its greatest activity at pH5.0 and temperature 40.0℃, and lost its activity quickly when temperature went above went 60℃. The POD activity increased fast when H2O2 was below 0.4%, and increased a little afterwards, reached the maximum at the H2O2 level of 0.6%. The order of combining capability of POD to various phenolic substrates was in the order of PPA>Chlorgenic acid>Guaiacol> Catechol>Tyrosine and Phenol.

Key words: Artemisia selengensis, fresh-cut, POD(Peroxidase), characteristics