关键词: 马铃薯, 转基因成分, 多重PCR

Abstract: The total DNAs of fifteen potatoes and their product samples were extracted by CTAB method. The PCR detection results of endogenous PATA gene were all positive, which showed that the DNAs were extracted successfully. The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase (nos) terminator and Escherichia coli strain K12 neomycin phosphotransferase II (nptⅡ) gene in these samples were all negative. The multiplex PCR detection methods of endogenous PAPA gene, Cauliflower mosaic virus (CaMV) 35S promoter, nos terminator and nptⅡ gene were developed, using the mixture of potato DNA and positive plamid pBI121 as PCR template. The multiplex PCR has some traits such as saving reagent and time, so it may be popularized to detect genetically modified products.

Key words:  , potato； genetically modified component； multiplex PCR；