食品科学 ›› 2006, Vol. 27 ›› Issue (1): 61-64.

• 基础研究 • 上一篇    下一篇

桃果实成熟软化过程中α-L-阿拉伯呋喃糖苷酶活性和乙烯合成相关因子的变化

金昌海,阚娟,索标,汪志君,陆兆新,郁志芳   

  1. 扬州大学食品科学与工程学院; 南京农业大学食品科技学院
  • 出版日期:2006-01-15 发布日期:2011-09-05

Changes of α-L-Arabinofuranosidase Activity and Relevant Factors of Ethylene Biosynthesis during Peach Ripening and Softening

JIN Chang-hai1,KAN Juan,SUO Biao,WANG Zhi-jun,LU Zhao-xin,YU Zhi-fang   

  1. 1.College of Food Science and Engineering, Yangzhou University; 2.College of Food Science and Technology, Nanjing Agricultural University
  • Online:2006-01-15 Published:2011-09-05

摘要: 以‘雨花三号’水蜜桃果实为试材,采用气相色谱法测定了桃果实成熟过程中乙烯释放量、氨基环丙烷-1-羧酸(ACC)氧化酶活性和ACC含量的变化,同时分析了α-L-阿拉伯呋喃糖苷酶(α-Afa)活性的变化。探讨了乙烯合成的相关因子和α-Afa对桃果实成熟软化的影响。结果表明:在桃果实成熟软化过程中,乙烯释放量、ACC氧化酶活性和ACC含量同时于成熟度Ⅳ出现高峰,α-Afa活性在成熟前期变化平缓,而在成熟中后期明显上升。本实验初步探明了α-Afa与乙烯的相互作用关系及其对桃果实成熟后期快速软化的作用机理。

关键词: 桃, 果实软化, 乙烯, 氧化酶, ACC, &alpha, -L-阿拉伯呋喃糖苷酶

Abstract:  A peach cultivar“ yuhuasanhao” was used to investigate by GC the changes of the ethylene production, ACC (1-aminocyclo propane-1-carboxyli acid) content and the activity of ACC oxidase in five different maturity stages. At the same time the changes of α-L-Arabinofuranosidase activity were analyzed. Their effect on peach softening was discussed. It was found that the time peak of ethylene was similar to that of ACC content and ACC oxidase. The activity of α-L-Arabinofuranosidase changed gently at initial stage and after that it experienced an increasing stage. In this paper, the result preliminary showed that the affecting mechanism of α-L-Arabinofuranosidase and ethylene on peach ripening and softening.

Key words:  , peach; softening; ethylene; oxidase; ACC(1-aminocyclo propane-1-1carboxylic acid); &alpha, -L-Arabinofuranosidase;