C30-HPLC-PDA分离与鉴定虾青素类化合物初探

食品科学 ›› 2006, Vol. 27 ›› Issue (10): 323-326.

C30-HPLC-PDA分离与鉴定虾青素类化合物初探

裴凌鹏, 惠伯棣, 刘蕊, 李京

中国中医科学院；北京联合大学应用文理学院；首都师范大学生命科学院

出版日期:2006-10-15 发布日期:2011-11-16

A Preliminary Investigation into the Separation and Identification of Astaxanthin and Related Compounds by C30-HPLC-PDA

PEI Ling-Peng, HUI Bo-Di, LIU Rui, LI Jing

1.China Academy of Chinese Medicine Science, Beijing 100700, China; 2.College of Applied Arts and Science, Beijing Union University, Beijing 100083, China; 3.College of Life Science, Capital Normal University, Beijing 100037, China

Online:2006-10-15 Published:2011-11-16

摘要/Abstract

摘要： 虾青素是一种在食品和药品领域应用广泛的具有预防和治疗疾病的功能因子。由于它在RP-HPLC色谱上难以形成良好的色谱峰形,虾青素及其相关化合物的分离和检测成为一项有难度的工作。本项研究中,应用C30-HPLC-PDA做了一系列工作来分离虾青素类化合物,主要包括:虾青素与其双酯的分离、虾青素几何异构体以及降解产物的分离。实验结果表明:在酸性乙腈-水(流动相A)和MTBE(流动相B)的流动相中,可以获得良好的分离效果。这一结果使应用C30-HPLC-PDA定量分析虾青素类化合物成为可能。

关键词: 虾青素, C30柱, HPLC

Abstract: Astaxanthin was an important functional factor wild applied in food and pharmaceutical products with various disease-protection and disease - treatment functions. The analysis of astaxanthin and related compounds was a difficult task due to their poor peak shapes on RP-HPLC. In this study, a series of effort was made to develop a separation method of astaxanthin- like compounds on C30-HPLC-PDA, including the separations of astaxanthin and its di-esters, geometrical isomers and degradation products. Data from those experiments suggested that by acidic acetonitrile-water (mobile phase A) and MTBE (mobile phase B), fractions mentioned as above were able to be separated successfully. This result is going to form the possibility of their quantization on C30-HPLC-PDA.

Key words: , astaxanthin； C30 column； HPLC；

裴凌鹏, 惠伯棣, 刘蕊, 李京. C30-HPLC-PDA分离与鉴定虾青素类化合物初探[J]. 食品科学, 2006, 27(10): 323-326.

PEI Ling-Peng, HUI Bo-Di, LIU Rui, LI Jing. A Preliminary Investigation into the Separation and Identification of Astaxanthin and Related Compounds by C30-HPLC-PDA[J]. FOOD SCIENCE, 2006, 27(10): 323-326.

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