食品科学 ›› 2006, Vol. 27 ›› Issue (11): 105-108.

• 基础研究 • 上一篇    下一篇

荧光假单胞菌AR4在2-酮基-D-葡萄糖酸工业生产中的应用研究

 孙文敬,  陈丽,  任蓓蕾,  张建国,  刘敬泽   

  1. 河北师范大学生命科学学院; 郑州拓洋实业有限公司
  • 出版日期:2006-11-15 发布日期:2011-11-28

Study on the Application of Phage-resistant Strain Pseudomonas fluorescens AR4 in Industrial Production of 2-keto-D-gluconic Acid

 SUN  Wen-Jing,   Chen-Li,   Ren-Bei-Lei,   Zhang-Jian-Guo,   Liu-Jing-Ze   

  1. 1.College of Life Science, Hebei Normal University, Shijiazhuang 050016, China; 2.Zhengzhou Tuoyang Industrial Co. Ltd., Zhengzhou 450001, China
  • Online:2006-11-15 Published:2011-11-28

摘要: 在噬菌体存在的情况下,采用抗噬菌体菌株荧光假单胞菌(Pseudomonasfluorescens)AR4在200m3发酵罐上进行了2-酮基-D-葡萄糖酸的发酵生产。结果表明,荧光假单胞菌AR4对生产环境中噬菌体的抗性稳定,发酵周期短,发酵转化率高;经过10次传代,荧光假单胞菌AR4对噬菌体的抗性及其发酵生产2-酮基-D-葡萄糖酸的能力没有改变;该菌株的发酵产物可以用于食品抗氧化剂D-异抗坏血酸钠的合成。

关键词:  , 2-酮基-D-葡萄糖酸, 荧光假单胞菌, 噬菌体, 抗噬菌体菌株, D-异抗坏血酸钠

Abstract:  In the presence of phages, 2-keto-D-gluconic acid fermentation by Pseudomonas fluorescens AR4, which was a phage- resistant mutant obtained from the mutation of the strain A46, was conducted in 200m3 fermentor. The results showed that the mutant resistance against the phages was stable, with shorter fermentation period and higher fermentation conversion rate than its parent. After 10 generations, its resistance against the phages and 2-keto-D-gluconic acid producing ability had not changed. The fermentation product of P. fluorescens AR4 could be used in the synthesis of sodium D-isoascorbate.

Key words:  , 2-keto-D-gluconic acid; Pseudomonas fluorescens; phage; phage-resistant mutant; sodium D-isoascorbate;