食品科学 ›› 2006, Vol. 27 ›› Issue (11): 395-396.

• 工艺技术 • 上一篇    下一篇

配位吸附柱层析法对卵磷脂分离纯化的研究

 朱骤海, 何俊婷   

  1.  陕西新药技术开发中心
  • 出版日期:2006-11-15 发布日期:2011-11-28

Separation of Phosphatidyl Cholines by Coordination Adsorption Column Chromatography

 ZHU  Zhou-Hai, HE  Jun-Ting   

  1. Shaanxi Pharmaceutical Development Center,Xi’an 710075,China
  • Online:2006-11-15 Published:2011-11-28

摘要: 采用配位吸附柱层析法分离纯化市售粉末大豆磷脂(磷脂酰胆碱含量40%),得到高纯度卵磷脂产品(磷脂酰胆碱含量96.2%)。重点建立了Cu2+型配体交换树脂柱,并优化了分离纯化条件。结果表明,该方法能简便有效地分离出高纯度卵磷脂。

关键词: 配位吸附柱层析, Cu2+型配体交换树脂, 卵磷脂

Abstract:  Phosphatidyl choline of high purity (PC content 96.2%) was prepared from commerial soybean powder phospholipids (PC content 40.0%) by coordination adsorption column chromatography (CACC). As the main objective,Cu2+ ligand exchange resin column was established for the separation of phosphatidyl cholines and CACC conditions were optimized. The results showed that,phosphatidyl choline of high purity could be effectively and facilely separated under the optimum CACC conditions.

Key words: coordination adsorption column chromatography, Cu2+ ligand exchange resin, phosphatidyl choline