食品科学 ›› 2006, Vol. 27 ›› Issue (12): 331-334.

• 工艺技术 • 上一篇    下一篇

加镧液体培养灰树花富集有机锌的研究

刘伟民,孙琳洁,刘姜,李国文   

  1.  江苏大学食品与生物工程学院; 江苏大学食品与生物工程学院
  • 出版日期:2006-12-15 发布日期:2011-11-23

Study on Zinc Accumulation of Grifola frondosus Cultured in Liquid with Lanthanide and Zinc

LIU Wei-min1,2,SUN Lin-jie1,LIU Jiang1,LI Guowen   

  1. 1.College of Food and Bioengineering, Jiangsu University, Zhenjiang 212013, China; 2.Jiangsu Produce Biologic Process and Separation Engineering Center, Zhenjiang 212013,China
  • Online:2006-12-15 Published:2011-11-23

摘要: 灰树花是药食两用真菌,富含多糖、氨基酸和多种矿物元素。利用灰树花将无机锌转化为有机锌,能为人体补锌提供一条新的有效途径。稀土元素对生物的生长代谢具有促进作用。为了研究镧和锌元素存在时灰树花液体培养富集锌的能力,设计了四因素四水平的正交试验方案。其因素和水平分别为:硝酸镧浓度50、100、150、200(mg/L培养基),1L培养基中碳源马铃薯/葡萄糖150/18、200/24、250/30、300/36(g/g),氮源麸皮20、25、30、35(g/L培养基)和7水硫酸锌浓度100、600、1300、2000(mg/L培养基)。分别以干菌丝有机锌含量、25ml培养基有机锌产量和锌的转化率3个指标进行了分析,并分别给出了其最优培养条件,以用于指导灰树花液体培养基的设计。以25ml培养基有机锌产量为指标时,得到优化培养基为1L培养基中含ZnSO4·7H2O2000mg、麸皮35g、La(NO3)350mg、马铃薯300g、葡萄糖36g。优化培养基下有机锌的产量为1.472mg/25ml,即58.88mg/L培养基。

关键词: 灰树花, 液体培养, 锌,

Abstract: Grifola frondosus is an edible and medicinal mushroom with rich polysaccharides ,amino acids and mineral elements. It is a new effective way to supply zinc to human body that Grifola frondosus is used to transfer inorganic zinc to organic zinc what can be easily absorbed by human body. The rare earth element lanthanide has effect of promoting on the growth of Grifola frondosus. For studying the ability of Grifola frondosus to accumulate zinc when cultured in liquid with the rare earth element lanthanide, authors designed a orthogonal test schemes which contained 4 factors and 4 levels. The factors and levels were as follows: La(NO3)3 concentration was 50, 100, 150, 200(mg/L culture medium); potato/glucose 150/18, 200/24, 250/30, 300/36 (g/g, 1L culture medium); bran 20, 25, 30, 35(g/L); ZnSO·47H2O concentration 100, 600, 1300, 2000(mg/L culture medium). The authors have used four criteria to analyze the experiments results and have obtained the optimum culture conditions for zinc amount in dry biomass, organic zinc yield and the zinc translation rate in order to guide technology design. With organic zinc yield in 25ml culture medium as criterion, the 1L optimal culture medium consists of ZnSO·47H2O 2000mg, bran 35g, La(NO3)3 50mg, potato 300g, glucose 36g. The organic zinc yield in 25ml culture medium is 1.472mg/25ml, that is 58.88mg/L culture medium.

Key words: Grifola frondosus, liquid culture, Zinc, lanthanide