食品科学 ›› 2006, Vol. 27 ›› Issue (2): 238-241.

• 分析检测 • 上一篇    下一篇

AccQ·Tag法测定绿豆蛋白酶解液中的氨基酸含量

  卢珍华, 郭彩华   

  1. 集美大学生物工程学院
  • 出版日期:2006-02-15 发布日期:2011-09-05

The RP-HPLC Determination of Amino Acids in Mung Bean Protein Hydrolysate by AccQ ·Tag

   Lu-Zhen-Hua, GUO  Cai-Hua   

  1. Bioengineering College, Jimei University, Xiamen 361021, China
  • Online:2006-02-15 Published:2011-09-05

摘要: 采用柱前衍生高效液相色谱法(AccQ·Tag法)同时测定17种氨基酸,氨基酸浓度在10~100μmol/L(胱氨酸浓度在5~50μmol/L)时,其峰面积和氨基酸浓度的线性相关系数均在0.99以上,17种氨基酸的加标回收率在92.1%~103.7%之间,用此法测定了绿豆蛋白酶解液氨基酸的含量,取得了满意的结果。

关键词: 高效液相色谱法, 氨基酸, AccQ·, Tag方法, 酶解液

Abstract: 17 amino acids were determined by HPLC AccQ ·Tag. When the concentration of amino acids were 10 ~100μmol/ L (with the cysteine among 5 ~50μmol/L), the correlation coefficient between the peak area and the concentration of amino acid was above 0.99. The recoveries of 17 amino acids added were 92.1% ~103.7%. The satisfactory method applied to amino acids in many bean protein hydrolysate and good results was obtained.

Key words:  , HPLC; amino acid; AccQ·, Tag; the soluble from mung bean protein;