万寿菊干花中叶黄素的实验室制备

食品科学 ›› 2006, Vol. 27 ›› Issue (6): 157-160.

万寿菊干花中叶黄素的实验室制备

惠伯棣, 唐粉芳, 裴凌鹏, 廖萍泰, 李京

北京联合大学应用文理学院；中国中医研究院；兰州医学院公共卫生学院；首都师范大学生命科学学院

出版日期:2006-06-15 发布日期:2011-09-22

Laboratory-scale Preparation of Lutein from Marigold Dried Flower

HUI Bo-Di, TANG Fen-Fang, PEI Ling-Peng, Liao-Ping-Tai, Li-Jing

1.College of Applied Arts and Science, Beijing Union University, Beijing 100083, China;2.China Academy of Traditional Chinese Medicine, Beijing 100009, China;3. Faculty of Public Health, Lanzhou Medical University, Lanzhou 730000, China;4. College of Life Science, Capital Normal University, Beijing 100037, China

Online:2006-06-15 Published:2011-09-22

摘要/Abstract

摘要： 用正己烷萃取万寿菊干花粉末多遍至萃取液无色,合并过滤萃取液。负压旋转蒸发浓缩萃取液至其中总类胡萝卜素含量为5%(W/V)。向浓缩液中加入等体积的10%(W/V)氢氧化钾乙醇溶液,在室温条件下,充氮,搅拌,皂化12h。用乙醚和2%(W/V)的氯化钠水溶液液—液萃取皂化反应产物。用氯化钠溶液多次洗涤乙醚相至氯化钠洗液的pH为7。收集乙醚相,负压旋转蒸发除去其中的乙醚,并进一步负压加热干燥除去水分至恒重。测定产物的总类胡萝卜素含量及类胡萝卜素组成。收集色谱图上叶黄素组分的质谱图作为对其定性的依据。

关键词: 万寿菊, 叶黄素

Abstract: The dried powders of marigold flower were extracted by hexane for a few times until the extract was colorless. After filtration, the extract was collected and concentrated by a rotary evaporator until its total carotenoid amount (TCA) was 5% (W/ V). The concentrate was saponified by the addition of an equal volume of 10% (W/V) KOH-EtOH. This reaction usually took more than 12 hours at room temperature with stirring and N2 filling. The product of saponification was isolated by a liquid-liquid phase separation with the upper phase of di-ether and the lower phase of 2% (W/V) NaCl solution. The ether phase was washedby the NaCl solution for many time until its pH was 7. The ether phase was then evaporated to remove di-ether and water. The TCA and carotenoid composition (CC) assessments of final concentrate were performed. A mass spectroscopy of lutein fraction was undertaken to support its identification by HPLC.

Key words: , marigold； lutein；

惠伯棣, 唐粉芳, 裴凌鹏, 廖萍泰, 李京. 万寿菊干花中叶黄素的实验室制备[J]. 食品科学, 2006, 27(6): 157-160.

HUI Bo-Di, TANG Fen-Fang, PEI Ling-Peng, Liao-Ping-Tai, Li-Jing. Laboratory-scale Preparation of Lutein from Marigold Dried Flower[J]. FOOD SCIENCE, 2006, 27(6): 157-160.

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