食品科学 ›› 2007, Vol. 28 ›› Issue (1): 126-130.

• 工艺技术 • 上一篇    下一篇

甘薯茎叶中绿原酸提取方法的研究及含量测定

 向昌国, 李文芳, 聂琴, 刘清波, 夏琴   

  1. 吉首大学张家界校区旅游学院; 吉首大学城乡资源与规划学院; 吉首大学城乡资源与规划学院 湖南张家界427000; 湖南张家界427000;
  • 出版日期:2007-01-15 发布日期:2011-12-31

Extracting Chlorogenic Acid from Sweet Potato Leaves and Stems and Determinating Its Content

 XIANG  Chang-Guo, LI  Wen-Fang, NIE  Qin, LIU  Qing-Bo, XIA  Qin   

  1. 1.College of Tour, Zhangjiajie Campus, Jishou University, Zhangjiajie 427000, China; 2.College of Resources and Planning Sciences, Jishou University, Zhangjiaji 427000, China
  • Online:2007-01-15 Published:2011-12-31

摘要: 甘薯是我国广泛种植的粮食作物之一,其茎叶的利用率很低。通过比较试验得出,浸提+超声波法适合甘薯叶、茎中绿原酸的提取。最佳提取条件是pH4,50%乙醇或甲醇为提取溶剂,浸提时间1.0~1.5h,温度40℃。分别对6~10月品种为86-21甘薯叶、茎中的绿原酸进行了提取,用紫外分光光度计进行了含量测定。结果表明9月份该品种叶和老茎中绿原酸含量最高,分别为:4.49%、1.26%,嫩茎7月份含量最高(0.93%),说明甘薯叶可以作为提取绿原酸的原材料,最佳采集时期为9月。该研究为甘薯叶、茎的开发利用和对其绿原酸的提取提供了一定的科学依据。

关键词: 甘薯茎叶, 绿原酸, 紫外分光光度法

Abstract: This paper reported the extraction and the determination of chlorogenic acid in sweet potato leaves and stems by UV-spectrophotometry. The results showed the follows optimum extraction process: pH4, menstruum 50% ethanol or methanol and extracting time 1.0~1.5h. The contents of chlorogenic acid in the leaves and stems of sweet potato are highest in September and are respectively 4.49% and 1.26%. The contents in the tender stems are highest in July as 0.93%. It provides a theoretical basis for the utilization of sweet potato leaves and stems.  

Key words: sweet potato leaves and stems, chlorogenic acid, UV-spectropHotometry