食品科学 ›› 2007, Vol. 28 ›› Issue (1): 176-179.

• 生物工程 • 上一篇    下一篇

产共轭亚油酸德氏乳杆菌的诱变选育

 郭焱, 于国萍   

  1. 东北农业大学食品学院; 东北农业大学食品学院 黑龙江哈尔滨150030; 黑龙江哈尔滨150030;
  • 出版日期:2007-01-15 发布日期:2011-12-31

Production of Conjugated Linoleic Acids by Lactobacillus. delbrueckii Mutation Breeding

 GUO  Yan, YU  Guo-Ping   

  1. College of Food Science, North East Agricultural University, Harbin 150030, China
  • Online:2007-01-15 Published:2011-12-31

摘要: 以徳氏乳杆菌?永茄侵郑保保矗福?为出发菌株,经硫酸二乙酯、紫外线对细胞悬液进行诱变处理,筛选出一株共轭亚油酸高产菌株1616u,并优化其培养条件,在培养基初始pH值为5;培养温度为37℃;培养时间为30h时,发酵液中可积累共轭亚油酸平均为11.04μg/ml,最高可达11.61μg/ml。

关键词: 共轭亚油酸, 徳氏乳杆菌, 诱变选育

Abstract: Original strain, Lactobacillus. delbrueckii subsp. bulgaricus 1.1482 was mutated by ultraviolet radition and DES. Then a strain named 1616u, producing conjugated linoleic acid 1616u was obtained.The optimum conditions were found, original pH of culture material 5, culture temperature 37℃, and culture time 30h 11.61μg/ml is the highest with an average of 11.04μg/ml conjugated linoleic acid.

Key words:  , conjugated linoleic acid; Lactobacillus delbrueckii; mutation breeding;