食品科学 ›› 2007, Vol. 28 ›› Issue (1): 212-215.

• 生物工程 • 上一篇    下一篇

一株海藻糖合成酶产生菌的筛选及初步鉴定

 尚宏丽, 陈红漫   

  1. 沈阳农业大学生物技术学院; 沈阳农业大学生物技术学院 辽宁沈阳110161; 辽宁沈阳110161;
  • 出版日期:2007-01-15 发布日期:2011-12-31

Study on Screening of Trehalose Synthase——Producing Strain and Identification

 SHANG  Hong-Li, CHEN  Hong-Man   

  1. College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China
  • Online:2007-01-15 Published:2011-12-31

摘要: 由长白山温泉附近土壤筛选得到菌株编号SH-110菌,分别利用TLC方法、HPLC法来确定该菌株所产的胞内酶能够利用麦芽糖作为底物来合成海藻糖,确定该酶为海藻糖合成酶,通过对该菌生物学特征鉴定,生理生化特征分析,初步鉴定该菌属于芽孢杆菌属(Bacillussp)。

关键词: 海藻糖合成酶, 筛选, 鉴定

Abstract: A strain of trehalose synthase—producing named SH-110 was isolated from the soil of hot springs of Changbai mountain.The study adopted TLC, DNS, HPLC in order to study the strain to produce trehalose synthase. Based on identifi- cation through its biological characteristics and physio-biochemical analysis, it is considered to be a variety of Bacillus sp.

Key words: trehalose synthase, screening, identification