食品科学 ›› 2007, Vol. 28 ›› Issue (11): 75-80.

• 基础研究 • 上一篇    下一篇

反应型可可香料的氨基酸和还原糖的Plackett-Burman设计筛选

 吴跃, 罗昌荣, 陈正行   

  1. 江南大学食品学院; 华宝食用香精香料(上海)有限公司技术中心; 江南大学食品学院 江苏无锡214122; 上海201821; 江苏无锡214122;
  • 出版日期:2007-11-15 发布日期:2011-11-22

Determination of Key Amino Acids and Reducing Sugars Contribution to Cocoa Flavor by Placket-Burman Design

 WU  Yue, LUO  Chang-Rong, CHEN  Zheng-Xing   

  1. 1.School of Food Science and Technology, Southern Yangtze University, Wuxi 214122, China; 2.Technology Center of Huabao Food Flavor and Fragrance (Shanghai) Co. Ltd., Shanghai 201821, China
  • Online:2007-11-15 Published:2011-11-22

摘要: 本研究采用Plackett-Burman试验设计,选取缬氨酸、甘氨酸、苯丙氨酸、亮氨酸、酪氨酸、苏氨酸、丝氨酸、谷氨酸、精氨酸、葡萄糖、果糖11个可能的可可风味相关因子进行筛选。统计分析结果表明:苯丙氨酸、丝氨酸、亮氨酸、谷氨酸、精氨酸、甘氨酸对美拉德反应的可可风味产物中的几种重要的致香成分具有最显著的影响。未发现葡萄糖和果糖对可可风味有任何差异贡献。选择这5种氨基酸和两种还原糖制备反应型可可香料,产物进行感官分析,并用GC-MS分析其中的致香成分和含量,鉴定出52种成分。

关键词: Plackett-Burman设计, 氨基酸, 还原糖, 可可风味, 美拉德反应

Abstract: The Plackett-Burnam design was applied to screen out the key factors from the following eleven components of amino acids and reducing sugars, which would affect cocoa flavor. Statistical analyses were performed. It was found that phenylalanine, serine, leucine, glutamic acid, arginine, glycine were significant factors for contributing to cocoa flavor but not the two reducing sugars, after the mixtures of five amino acids and two reducing sugars were selected to react for the reaction cocoa flavors. Then these reacting products analysed by organoleptic investigation and GC-MS and 52 compounds are identified.

Key words: Placket-Burman design, amino acid, reducing sugar, cocoa flavor, maillard reaction