大马哈鱼鱼精蛋白的提取及抑菌作用的研究

食品科学 ›› 2007, Vol. 28 ›› Issue (2): 37-39.

大马哈鱼鱼精蛋白的提取及抑菌作用的研究

刘红玉, 姜学芬, 崔洪斌

哈尔滨医科大学公共卫生学院；黑龙江大通生物技术有限公司；哈尔滨医科大学公共卫生学院黑龙江哈尔滨150086；黑龙江哈尔滨150090；黑龙江哈尔滨150086；

出版日期:2007-02-15 发布日期:2011-12-31

Study on Extraction and Antimicrobial Activities of Protamine from Oncorhynchus Keta Spermary

LIU Hong-Yu, JIANG Xue-Fen, CUI Hong-Bin

1.Public Health College, Harbin Medical University, Harbin 150086, China; 2.Heilongjiang DaTong Biotechnology Co. Ltd., Harbin 150090, China

Online:2007-02-15 Published:2011-12-31

摘要/Abstract

摘要： 对从大马哈鱼精巢中提取鱼精蛋白的工艺条件及其抑菌活性进行了研究。采用正交试验法筛选提取鱼精蛋白的最佳工艺条件,其条件为:H2SO4浓度为7.5%,用量为鱼精的3.5倍,提取时间2h,95%乙醇用量为鱼精的3倍。对鱼精蛋白进行分析,其蛋白质含量达到89.7%,且精氨酸含量较高。该鱼精蛋白对革兰氏阴性菌、革兰氏阳性菌和酵母菌均具有较强的抑菌活性,可作为一种天然防腐剂。

关键词: 大马哈鱼, 鱼精蛋白, 抑菌活性

Abstract: Objective: The extraction process of protamine from Oncorhynchus Keta spermary and its antimicrobial activities were studied. The extraction conditions of extracting protamine were optimized by the orthogonal design: H2SO4 concentration 7.5%, volume H2SO4 3.5 times that of speractary volume of 95% alcohol solution 3 times that of spermary and extraction time 2 hours. The nutritive values of the protamine were analysed. The results showed that the protein content of protamine is 89.7%, and the content of arginine is even higher. The results of active test suggested that the protamine shows fairly high antimicrobial activity against gram-negative bacteria, gram-positive bacteria and Saccharomyces cerevisiae as well. It is an effectiven aturala ntiseptic.

Key words: Oncorhynchus Keta, protamine, antimicrobial activity

刘红玉, 姜学芬, 崔洪斌. 大马哈鱼鱼精蛋白的提取及抑菌作用的研究[J]. 食品科学, 2007, 28(2): 37-39.

LIU Hong-Yu, JIANG Xue-Fen, CUI Hong-Bin. Study on Extraction and Antimicrobial Activities of Protamine from Oncorhynchus Keta Spermary[J]. FOOD SCIENCE, 2007, 28(2): 37-39.

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