食品科学 ›› 2007, Vol. 28 ›› Issue (2): 53-55.

• 基础研究 • 上一篇    下一篇

黑木耳多糖的酶法提取、纯化及性质研究

 韩春然, 唐娟, 马永强   

  1. 哈尔滨商业大学食品工程学院; 哈尔滨商业大学食品工程学院 黑龙江哈尔滨150076; 黑龙江哈尔滨150076;
  • 出版日期:2007-02-15 发布日期:2011-12-31

Study on Isolation, Purification and Characteristics of Polysaccharides from Auricularia auricula

 HAN  Chun-Ran, TANG  Juan, MA  Yong-Qiang   

  1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
  • Online:2007-02-15 Published:2011-12-31

摘要: 本文以双酶法提取黑木耳粗多糖,利用DEAE-Cellulose 52和Sepharose CL-4B柱层析对其进行纯化,以Sephadex G-100凝胶柱层析测定了多糖的分子量。结果表明黑木耳多糖含有两个主要组分APE Ⅰ和APE Ⅱ,分子量分别为31.7×104和18.3×104,均为非淀粉类多糖,不含单糖、蛋白质、核酸及多酚类物质。

关键词: 黑木耳, 多糖, 层析, 纯化, 分子量

Abstract: The Auricularia auricular polysaccharides were extracted by proteinase and celluloase, purified by DEAE- Cellulose 52 and Sepharose CL-4B chromatography.The molecular weight was determined by Sephadex G -100 chromatograph. The results showed that there are two main fractions in Auricularia auricular polysaccharides with the molecular weight of 31.7×104 and 18.3×104, respectively, which are non-starch polysaccharides, free of simple sugar, protein, nuclear acid and phenols.

Key words: Auricularia auricular, polysaccharides, chromatography, purify, molecular weight