食品科学 ›› 2007, Vol. 28 ›› Issue (4): 175-178.

• 工艺技术 • 上一篇    下一篇

锦橙键合态芳香物质酶解条件优化研究

 陈朸为, 潘思轶   

  1. 华中农业大学食品科技学院; 华中农业大学食品科技学院 湖北 武汉 430070; 湖北 武汉 430070;
  • 出版日期:2007-04-15 发布日期:2011-12-31

Study of Optimization on Bound Volatile Aroma Compounds Hydrolyzed byβ-D-glucosidase in Glorious Oranges Juice

 CHEN  Li-Wei, PAN  Si-Yi   

  1. College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Online:2007-04-15 Published:2011-12-31

摘要: 研究了锦橙键合态芳香物质的酶解条件。锦橙果汁通过Amberite XAD-2树脂柱分离得到键合态芳香物质,通过正交试验确定Almondβ-D-葡萄糖苷酶酶解键合态芳香物质的最佳酶解条件,酶解后的芳香成分采用GC和GC-MS进行定性定量分析。结果表明:酶解最佳条件是温度40℃,时间24h,酶用量为200U。

关键词: 锦橙, 键合态芳香物质, 酶解条件, 优化

Abstract: Optimization of bound volatile aroma compounds hydrolyzed byβ-D-glucosidase in Glorious Oranges juice of Orange was studied.The bound volatile aroma compounds were absorbed and separated by AmberiiteXAD-2 from Glorious Oranges,and optimum of hydrolysis of bound volatile aroma compounds byβ-D-glucosidase was studied by orthogonal test. The volatile aroma compounds were analyzed by GC and GC-MS.The result shows that optimum conditions are:enzymatic hydrolysis temperature 40℃,the enzymatic hydrolysis time 24h and the enzymatic quantity 200U.

Key words: Glorious Oranges, bound volatile aroma compounds, enzymatic hydrolysis, optimization