食品科学 ›› 2007, Vol. 28 ›› Issue (4): 208-211.

• 生物工程 • 上一篇    下一篇

响应面法优化以豆渣为基质发酵纳豆菌

 孙艳辉, 董英   

  1. 江苏大学食品与生物工程学院; 江苏大学食品与生物工程学院 江苏 镇江 212013; 江苏 镇江 212013;
  • 出版日期:2007-04-15 发布日期:2011-12-31

Optimization of Bacillus natto Fermented from Soybean Dregs by Response Surface Methodology

 SUN  Yan-Hui, DONG  Ying   

  1. School of Food and Bioengineering,Jiangsu University,Zhenjiang 212013,China
  • Online:2007-04-15 Published:2011-12-31

摘要: 为提高豆渣的附加值,以豆渣为基质液体发酵纳豆菌;首先用Plackett-Burman方法对影响纳豆菌发酵因素的效应进行评价并筛选出了具有显著效应的三个因素:初始pH值、发酵温度和麸皮含量,其他因素对纳豆菌发酵无显著影响;然后采用最陡爬坡实验逼近最大响应区域,最后由响应面实验确定了主要影响因素的最佳条件:初始pH6.02、发酵温度36.4℃、麸皮0.31%;在优化培养条件下,纳豆菌发酵液活菌数达到4.35×10~9CFU/ml。

关键词: 豆渣, 纳豆菌, 微生态制剂, 响应面法

Abstract: In order to improve soybean dregs added value,soybean dregs were utilized as medium to ferment Bacillus natto. Firstly,a Plackett-Burman design was used to evaluate the affecting factors of Bacillus natto fermented.Initial pH value, fermentation temperature and bran content are significant factors.The others had no significant effect on fermenting Bacillus natto.The steepest ascent experiment is used to approach the optimal conditions subsequently.Finally the optimized conditions are confirmed initial pH value 6.02,fermentation temperature 36.4℃and bran 0.31% further optimized with response surface methodology.The best result is 4.35×10~9CFU/ml life bacteria count under the optimized conditions.

Key words: soybean dregs, Bacillus natto, probiotics, response surface methodology