食品科学 ›› 2007, Vol. 28 ›› Issue (6): 146-149.

• 工艺技术 • 上一篇    下一篇

以活化硒矿为硒源的灵芝生物富硒研究

 张连富, 窦春霞, 王莲芳, 胡蔚红   

  1. 江南大学食品学院食品科学与安全教育部重点实验室; 恩施州硒产品开发研究所 江苏无锡214036; 江苏无锡214036; 湖北恩施445000;
  • 出版日期:2007-06-15 发布日期:2011-12-31

Study on Selenium Accumulation in Ganoderma Lucidum with Activated Ore as Selenium Source

 ZHANG  Lian-Fu, DOU  Chun-Xia, WANG  Lian-Fang, HU  Wei-Hong   

  1. 1.Key Laboratory of Food Science and Security, Ministry of Education, School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, China;2.Enshi Selenium-containing Products Developing Institute, Enshi 445000, China
  • Online:2007-06-15 Published:2011-12-31

摘要: 对以活化硒矿为硒源的灵芝(Ganoderma lucidum)液体培养条件进行了研究。考察了不同碳源和氮源对灵芝生长的影响,利用正交试验确定了最佳液体发酵培养基并对温度、转速、pH等发酵条件进行了优化。试验结果表明:灵芝液体培养的最佳发酵培养基是:2%玉米粉,4%葡萄糖,3%麸皮汁,0.2%磷酸二氢钾,0.1%七水硫酸镁;最佳发酵培养条件是:温度28℃,摇床转速180r/min,pH6,接种量15%,菌丝生物量高达23.59g/L。当添加的硒矿水溶硒含量为5μg/g时,灵芝富硒效果最好,其总硒含量和富硒率分别达302.2μg/g和60.0%。

关键词: 硒矿, 富硒, 灵芝

Abstract: The submerged culture conditions of Ganoderma Lucidum with activated selenium-containing ore as selenium source were studied. The effects of different carbon and nitrogen sources on the growth of Ganoderma Lucidum were investigated and the optimal fermenting conditions including temperature, rotating speed and pH of the medium were determined.The results showed that the optimal constitutes of the culture medium are: 2% maize meal, 4% glucose, 3% bran juice, 0.2% KH2PO4 and 0.1% MgSO·47H2O, and the optimal fermenting conditions are as follows: 28 ℃, 180 r/min, pH 6 and 15% inoculum volume. The dry weight of Ganoderma Lucidum is 23.59 g/L. When the selenium content of the culture is chosen as 5 μg/g, the total selenium content in Ganoderma Lucidum reaches 302.2μg/g and the selenium accumulation rate is 60.0%.

Key words: selenium ore, selenium accumulation, Ganoderma lucidum