食品科学 ›› 2007, Vol. 28 ›› Issue (7): 242-244.

• 工艺技术 • 上一篇    下一篇

超滤法分离免疫牛初乳中的IgG

 范丽娟, 潘道东   

  1. 南京师范大学金陵女子学院食品科学与营养系; 南京师范大学金陵女子学院食品科学与营养系 江苏南京210097; 江苏南京210097;
  • 出版日期:2007-07-15 发布日期:2011-10-24

Seperation of IgG from Immune Bovine Colostrum by Ultrafiltration

 FAN  Li-Juan, PAN  Dao-Dong   

  1. Department of Food Science and Nutrition, Ginling College, Nanjing Normal University, Nanjing 210097, China
  • Online:2007-07-15 Published:2011-10-24

摘要: 本实验选用截留分子量为100kD的中孔纤维超滤膜从免疫牛初乳乳清中分离提取IgG,研究了操作压力和温度在超滤过程中对膜透过速率的影响,确立了最适操作压力为0.15MPa,最适操作温度为25℃;并采用分段超滤法对免疫牛初乳乳清中的IgG进行分离,从而使浓缩液中IgG纯度得到进一步提高。

关键词: 免疫牛初乳, IgG, 超滤分离

Abstract: IgG was separated and extracted from immune bovine colostrums whey by hollow-fiber membrane ultrafiltration (100 kD, molecular, cut-off). The factors affecting separation such as pressure and temperature were studied. The results showed that optimum operating pressure and temperature were 0.15 MPa and 25 ℃ respectively, and the purity of IgG in concentrated liquid could be further improved by subsection ultrafiltration.

Key words: immune bovine colostrums, IgG, ultrafiltration sepration