食品科学 ›› 2007, Vol. 28 ›› Issue (9): 158-161.

• 工艺技术 • 上一篇    下一篇

山药多糖的分离纯化及组成研究

 顾林, 姜军   

  1. 扬州大学食品科学与工程学院; 扬州大学食品科学与工程学院 江苏扬州225001; 江苏扬州225001;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Study on Constitutes,Separation and Purification Technology of Yam Polysoccharides

 GU  Lin, JIANG  Jun   

  1. College of Food Science and Engineering,Yangzhou University,Yangzhou 225001,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 本实验研究了山药多糖的提取工艺,得出最佳工艺条件为料液比1:25,100℃下浸提2.5h,此时山药多糖的提取率最大。在进一步的利用DEAE52,Sephadex G-100分离纯化中,我们发现了三种多糖,包括中性多糖和两种酸性多糖。利用GC-MS分析结果显示,三种多糖的单糖组成分别为:中性糖由葡萄糖和甘露糖组成,酸性多糖1是由葡萄糖、半乳糖、甘露糖组成。酸性糖2是由阿拉伯糖、木糖、阿卓糖、葡萄糖、甘露糖组成。

关键词: 多糖, 纯化, 气质联用色谱

Abstract: This paper studied the separation technics of the yam polysaccharides.The results showed the optimum conditions to separate the polysaccharide are:S/W 1:25,tempetrature 100 ℃ and time 2.5 h.During the next purification step,three kinds of polysaccharides are found,including one neutral polysaccharide and two acid polysaccharides.The GC-MS results showed that the neutral polysaccharide includes glucose and mannose,but acid polysaccharide 1 includes glucose,mannose and galactose,acid polysaccharide 2 includes glucose,mannose arabinose,xylose and altrose.

Key words: polysaccharide, purification, GC-MS