关键词: 胡麻卵磷脂, 磷脂酰胆碱, 柱层析, 薄层层析

Abstract: This research take the powdery Xinjiang flax phosphatide as the raw material,obtains the high-purity lecithin after applying the methods of metallic ion precipitation method,column chromatographic analysis,entire solvent extraction method and thin layer chromatographic analysis.And it has studied the effect influence of metallic ion precipitation method on the separation of phosphatide mixture by the columns chromatographic analysis.The result indicated that,take the metallic ion zinc as the compound precipating ion,and the neutral aluminum oxide as the absorbent,take chloroform:methyl alcohol:water=65:25:4(V:V:V)to elutes the medicinal preparation,the high-purity lecithin can be obtained;the phospho-lipin mixture without the metallic ion precipitation is unable to separate the sole component lecithin,regarding this part of phospho-lipin mixture,the research uses the thin layer chromatographic analysis to obtain the pure lecithin.The obtained lecithin has been distinguisded by the infrared spectrum,the ultra-violet spectrograph,one-dimensional launches the thin layer chromatographic analysis,two-dimensional double fluid launches method and so on.

Key words: flax lecithin, phosphatidyl choline, column chromatographic analysis, thin layer chromatographic analysis