食品科学 ›› 2007, Vol. 28 ›› Issue (9): 271-274.

• 工艺技术 • 上一篇    下一篇

丁醇沉淀法分离粗直链淀粉的研究

 高炜丽, 赵燕, 李建科   

  1. 南昌大学食品科学教育部重点实验室; 南昌大学食品科学教育部重点实验室 江西南昌330047; 江西南昌330047;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Study on Separating Coarse Amylose by Butanol Precipitation

 GAO  Wei-Li, ZHAO  Yan, LI  Jian-Ke   

  1. Key Laboratory of Food Science,Ministry of Education,Nanchang University,Nanchang 330047,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 以普通玉米淀粉为原料,根据直链淀粉和支链淀粉的结构特性不同,采用丁醇沉降法分离直链淀粉粗产品,讨论了分离过程中离心温度,离心力大小等对分离效果的影响,得出在8000r/min,2℃时离心15min分离效果最佳,而且通过对淀粉黏度、蓝值、分子量分布的测定及与标准品的比较,证实该法分离的直链淀粉纯度较高。

关键词: 丁醇沉淀, 分离, 直链淀粉, 影响因素

Abstract: In this paper,according to the structural property and physicochemical property of amylose and amylopetin,we use butanol precipitation separating amylose and amyloptin from common corn starch.Disscussed the effect of centrifugal time,centrifugal force on separating efficiency of amylose and amylopetin.The resultes showed that at 2 ℃,8000 r/min,centrifuge 15 mins,we could get the best separate efficiency,and at last we compared the viscosity,blue value,gel permeation chromatog-raphy of the amylose sample with the standard amylose,confirmed the coarse amylose had a high purity.

Key words: butanol precipitation, separating, amylose, effect factors