食品科学 ›› 2007, Vol. 28 ›› Issue (9): 315-318.

• 工艺技术 • 上一篇    下一篇

白葡萄酒废酵母蛋白及多糖的提取工艺研究

 屈慧鸽, 于小飞, 张玉香, 程显好, 王晓玲, 杨建志   

  1. 鲁东大学生命科学学院; 鲁东大学生命科学学院 山东烟台264025; 山东烟台264025;
  • 出版日期:2007-09-15 发布日期:2011-11-22

Study on Extraction Techniques of Protein and Polysaccharide from Waste Yeast of White Wine

QU  Hui-Ge, YU  Xiao-Fei, ZHANG  Yu-Xiang, CHENG  Xian-Hao, WANG  Xiao-Ling, YANG  Jian-Zhi   

  1. College of Life Science,Ludong University,Yantai 264025,China
  • Online:2007-09-15 Published:2011-11-22

摘要: 本实验主要研究了从白葡萄酒废酵母中提取蛋白和多糖的方法。结果表明:经处理后的酵母悬浮液在超声波功率600W,作用时间4s,间歇时间2s,作用90次的条件下,促溶效果最好。经超声波处理后,再添加0.6%(W/V)的木瓜蛋白酶,在55℃,pH5.5下,反应时间为30h,提取液中蛋白含量达到9.84g/L。提取蛋白后的残渣,用碱法提取多糖,含量达58.69%。

关键词: 白葡萄酒废酵母, 蛋白, 多糖, 超声波, 木瓜蛋白酶

Abstract: The extracted techniques of protein and polysaccharide from white wine waste yeast were studied.The results showed:The best disaggregated effect of yeast suspension by ultrasonic is disposed in 600W,acting 4s,interval 2s,90 times.Then,put 0.6% papain into the yeast suspension which treat with ultrasonic,pH 5.5,55 ℃,reaction 28 h,the protein content of the extraction was 9.84 g/L.The polysaccharide obtained from the residue which extracted protein by NaOH solution.The polysaccharide content of extraction was 58.69%.

Key words: white wine waste yeast, protein, polysaccharide, ultrasonic, papain