食品科学 ›› 2008, Vol. 29 ›› Issue (10): 698-705.

• 专题论述 • 上一篇    下一篇

转基因食品检测技术的应用与发展Ⅰ.主要检测技术及其特点

 王广印, 范文秀, 陈碧华, 张建伟, 韩世栋   

  1. 河南科技学院园林学院; 河南科技学院化学化工学院; 河南科技学院实验中心; 潍坊职业学院;
  • 出版日期:2008-10-15 发布日期:2011-12-08

Application and Development of Detection Technology of Genetically Modified Foods (GMFs) Ⅰ. Main Detection Technologies of GMFs and Their Characteristics

 WANG  Guang-Yin, FAN  Wen-Xiu, CHEN  Bi-Hua, ZHANG  Jian-Wei, HAN  Shi-Dong   

  1. 1.College of Landscape,Henan Institute of Science and Technology,Xinxiang 453003,China;2. School of Chemistry and Chemical Engineering,Henan Institute of Science and Technology,Xinxiang 453003,China;3.Experimental Center,Henan Institute of Science and Technology,Xinxiang 453003,China;4.Weifang Vocational College,Weifang 261041,China
  • Online:2008-10-15 Published:2011-12-08

摘要: 本文概述了包括Western印迹法、酶联免疫吸附实验法、聚合酶链反应法、Southern印迹法等在内的蛋白质检测和DNA检测方法,并介绍了DNA微阵列技术法等新方法。对这些方法的基本原理、优缺点和应用范围以及存在的问题进行了分析,可供转基因食品检测研究及选择分析方法时借鉴。

关键词: 转基因食品, 检测技术, PCR, ELISA

Abstract: A number of protein or DNA methods for detection of genetically modified foods (GMFs) were discussed in this review,including western blot,enzyme linked immunesorbent assay (ELISA),polymerase chain reaction (PCR),southern blot and so on. Several new methods have been recommended to detect genetically modified foods,such as microarray and PCR-ELISA. The discussions were made on various detection methods in terms of their principle,advantage and disadvantage,application and problems.

Key words: genetically modified foods (GMFs), detection technology, polymerase chain reaction(PCR), enzyme linked immunesorbent assay (ELISA)