食品科学 ›› 2008, Vol. 29 ›› Issue (11): 165-168.
• 工艺技术 • 上一篇 下一篇
徐蓉, 李燕
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XU Rong, LI Yan
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摘要: 通过对芦荟苷的静态吸附和动态洗脱实验,对6种大孔吸附树脂进行了筛选。结果表明:HZ-801型树脂吸附与洗脱效果最佳。优化出的最佳工艺参数为:在上样浓度为0.2765mg/ml的条件下,上样体积为520ml、洗脱剂60%乙醇、洗脱速度2ml/min、洗脱体积3倍柱床体积。纯化倍数为14.72,回收率86.3%。
关键词: 芦荟苷, 大孔吸附树脂, 分离纯化, 筛选
Abstract: By static adsorption and continuous elution experiments on aloin,the macroporous resin HZ-801 having the highest adsorption capacity and elution ratio was screened out of six macroporous resins.Its optimal purification process param-eters are determined as follows:extract solution at a concentration of 0.2765 mg/ml,adsorption volume 520 ml,60% ethanol as eluent,elution flow rate 2 ml/min,and eluent volume 3 BV(three times bed volume).Under these conditions,the purification fold is 14.72,and the recovery is 86.3%.
Key words: aloin, macroporous resin, separation and purification, screening
徐蓉, 李燕. 大孔吸附树脂纯化库拉索芦荟中芦荟苷的研究[J]. 食品科学, 2008, 29(11): 165-168.
XU Rong, LI Yan. Study on Purification of Aloin from Aloe barbadensis Miller with Macroporous Resin[J]. FOOD SCIENCE, 2008, 29(11): 165-168.
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