食品科学 ›› 2008, Vol. 29 ›› Issue (11): 379-382.

• 生物工程 • 上一篇    下一篇

枯草杆菌发酵生产VK2的技术工艺条件初探

 李拖平, 郭梅, 王娜, 陈积星   

  1. 辽宁大学食品科学系; 天津农学院食品科学系;
  • 出版日期:2008-11-15 发布日期:2011-12-08

Preliminary Study on Production Conditions of VK2 by Fermentation of Bacillus subtilis

 LI  Tuo-Ping, GUO  Mei, WANG  Na, CHEN  Ji-Xing   

  1. 1.Department of Food Science,Liaoning University,Shenyang 110036,China;2.Department of Food Science,Tianjin Agricultural University,Tianjin 300384,China
  • Online:2008-11-15 Published:2011-12-08

摘要: 对枯草杆菌发酵生产VK2的培养条件进行优化的结果表明,培养基中大豆蛋白和玉米淀粉的浓度过高或过低均不利于VK2的产生与积累,其最适浓度均为2%。甘油作为枯草杆菌的补助碳源对VK2的产生与积累具有一定的促进作用,其最适添加量为5%。1%蔗糖作为枯草杆菌生长的外源性糖源对VK2的生产具有明显的促进作用,但Ca2+、Mg2+、K+等一些无机离子对VK2的产生都表现出不同程度的抑制作用。

关键词: 枯草杆菌, VK2, 发酵条件

Abstract: This study aimed to incestigate the production conditions of vatamin K2(VK2) by Bacillus subtilis fermentation preliminarily.Results showed that higher or lower concentrations of soybean protein and cornstarch are disadvantage for the production of VK2.The optimal concentration of both soybean protein and cornstarch is 2%.Glycerol,as a supplementary carbon source,can promote the biosynthesis of VK2,and its effective concentration is 5%.1% sucrose,as an exogenous carbon source can obviously promote the B.subtilis fermentation to increase the productivity of VK2.Several inorganic ions such as Ca2 +,Mg2 + and K + represent inhibitive effects on the production of VK2.

Key words: Bacillus subtilis, vitamin K2, fermentation condition