食品科学 ›› 2008, Vol. 29 ›› Issue (12): 610-613.

• 分析检测 • 上一篇    下一篇

富硒平菇蛋白测定与氨基酸成分分析

 王新风, 杨芳, 刘圣师, 朱大伟   

  1. 淮阴师范学院资源微生物研究所; 淮阴师范学院化学系;
  • 出版日期:2008-12-15 发布日期:2011-12-08

Determination of Soluble Protein and Analysis of Amino Acid Compositions of Selenium(Se)-accumulated Pleurotus ostreatus

 WANG  Xin-Feng, YANG  Fang, LIU  Sheng-Shi, ZHU  Da-Wei   

  1. 1.Institute of Resource Microorganism,Huaiyin Teachers’ College,Huaian 22330,China; 2.Department of Chemistry,Huaiyin Teachers’ Colle ge,Huaian 22330,China
  • Online:2008-12-15 Published:2011-12-08

摘要: 目的:研究培养料添加10~50mg/kg不同浓度无机硒后,所采收的富硒平菇中蛋白的含量,并对菇体中硒含量为7~10μg/g干重(10mg/kg处理组),可直接上市供鲜食的富硒平菇组进行氨基酸成分分析。方法:考马斯亮蓝G-250法测定富硒平菇干粉的可溶性蛋白,凯氏定氮法测定总蛋白含量,氨基酸自动分析仪进行氨基酸成分分析。结果:富硒平菇干粉中可溶性蛋白含量都明显高于对照组,最高达对照的2.25倍;可直接上市供鲜食的富硒平菇体内的氨基酸成分分析:人体必需的7种氨基酸(色氨酸没测定)占氨基酸总量的47.44%,半必需氨基酸占氨基酸总量的14.39%,支链氨基酸占氨基酸总量的20.37%。结论:富硒平菇是一种具有较高的保健和药用开发价值的功能性产品。

关键词: 富硒平菇, 蛋白, 氨基酸

Abstract: Objective:To study content of protein in Se-accumulated Pleurotus ostreatus that added different concentrations from 10 to 50 mg/kg inorganic selenium to the culture and analyse component of amino acid in the 10 mg/kg Se-treated Pleurotus ostreatus that had content of 7 to 10 μg/g selenium/g dry body and could be sold directly and eaten safely. Method:Coomassie brilliant blue G-250 was used to determine content of soluble protein,Kjeltec Protein Analyzer was used to determine content of total protein,and amino acid auto analyzer was used to analyse amino acid composition. Results:The content of soluble protein in the attained powder of Se-accumulated Pleurotus ostreatus is evidently higher than that of the control,reaching 2.25 times; The powder of the 10 mg/kg selenium treated Pleurotus ostreatus has 7 human essential amino acids(except trptophane) accounting for about 47.44% of total amino acid,nonessential amino acid accounting for 14.39% of total amino acid and branched-chain amino acid accouting for 20.37% of total amino acid. Conclusion:Se-accumulated Pleurotus ostreatus is a functional product which has high health protection and medical utilization value.

Key words: Se-accumulated Pleurotus ostreatus, protein, amino acid