食品科学 ›› 2008, Vol. 29 ›› Issue (2): 218-224.

• 生物工程 • 上一篇    下一篇

内蒙古酸马奶中乳酸菌多样性的研究(英文)

 刘芳, 都立辉, 杜鹏, 霍贵成   

  1. 东北农业大学乳品科学教育部重点实验室; 东北农业大学乳品科学教育部重点实验室 黑龙江哈尔滨150030; 黑龙江哈尔滨150030;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Biodiversity of Lactic Acid Bacteria in Home-made Koumiss from Inner Mongolia

 LIU  Fang, DU  Li-Hui, DU  Peng, HUO  Gui-Cheng   

  1. Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 采用16S rRNA 基因全序列测定和聚类分析技术,对酸马奶中的乳酸菌进行了准确鉴定并构建了乳酸菌的系统发育树。然后对乳酸菌菌群进行了多样性分析,结果显示,酸马奶中的优势乳酸菌分别为:Lactobacillus plantarum(10%),Lactobacillus brevis(8%),Lactobacillus casei(7.8%),Enterococcus faecium(17%),Enterococcus faecalis(14%),Lactococcus lactis(19%),Lactobacillus acidlophilus(5%),Lactobacillus paracasei(2%),Lactobacillus delbrueckii subsp. bulgaricus(4%),Lactobacillus helveticus(4%),Enterococcus durans(4%),Leuconostoc mesenteroides(4%),Leuconostoc garlicum(1%),Streptococcus thermophilus(1%)。

关键词: 酸马奶, 乳酸菌, 生物多样性, 16S rRNA基因

Abstract: In order to identify and protect home-made Koumiss, the diversity of lactic acid bacteria was investigated using appropriate starter cultures. Analysis of the 16S rRNA gene was used to confirm the presence of the predominant groups indicated by phenotypic analysis and to determine the phylogenetic affiliation of representative strains. The prevalent lactic acid bacteria consisted of Lactobacillus plantarum(10%), Lactobacillus brevis(8%), Lactobacillus casei(7.8%), Enterococcus faecium (17%), Enterococcus faecalis(14%), Lactococcus lactis(19%), Lactobacillus acidlophilus(5%), Lactobacillus paracasei(2%), Lactobacillus delbrueckii subsp. bulgaricus(4%), Lactobacillus helveticus(4%), Enterococcus durans(4%), Leuconostoc mesenteroides(4%), Leuconostoc garlicum(1%) and Streptococcus thermophilus(1%).

Key words: Koumiss, lactic acid bacteria, biodiversity, 16S rRNA gene