食品科学 ›› 2008, Vol. 29 ›› Issue (2): 403-405.

• 营养卫生 • 上一篇    下一篇

五种抗氧化剂抗氧化活性的比较研究

 曹剑锋, 侯国鹏, 李嘉宏, 刘国安   

  1. 西北师范大学生命科学学院; 西北师范大学生命科学学院 甘肃兰州730070; 甘肃兰州730070;
  • 出版日期:2008-02-15 发布日期:2011-08-24

Study on Antioxidative Activity of Five Natural Products

 CAO  Jian-Feng, HOU  Guo-Peng, LI  Jia-Hong, LIU  Guo-An   

  1. College of Life Science, Northwest Normal University, Lanzhou 730070, China
  • Online:2008-02-15 Published:2011-08-24

摘要: 本实验采用甲基紫比色法、MDA法和SDS-PAGE,检测了五种天然产物清除羟自由基的能力、对脂质过氧化及蛋白质氧化的保护作用。结果表明,五种化合物在不同的反应体系表现出不同的抗氧化活性。茜素红清除·OH的能力及对蛋白质氧化的保护作用最强,白藜芦醇对脂质过氧化的抑制作用最强。同时实验结果也反映出化合物的溶解性对其抗氧化作用有很大的影响。

关键词: 天然产物, 羟自由基, 脂质过氧化, 蛋白质氧化, 抗氧化作用

Abstract: In this study, methyl violet assay, MDA determination and SDS-PAGE were used to investigate antioxidative activities of five natual products. The hydroxyl radical scavenging ability, protections against lipid peroxidation and protein oxidation were measured. The results showed that five natual products exhibited different antioxidative activities in different reactive systems. Alizarin red has the strongest effect in hydroxyl radical scavenging and protein oxidation protection. Resveratrol exhibit strongest inhibition to lipid peroxidation. The results also reflect the dissolve nature of compounds play a role in its antioxidation system.

Key words: antioxidation, hydroxyl radical, lipid peroxidation, protein oxidation, antioxidative activity