虫草多糖醇沉和DEAE-32纤维素柱层析特性研究

食品科学 ›› 2008, Vol. 29 ›› Issue (2): 86-90.

虫草多糖醇沉和DEAE-32纤维素柱层析特性研究

武忠伟, 刘明久, 窦艳萍, 赵现方

河南科技学院生物工程系；河南科技学院农学系；河南科技学院生物工程系河南新乡453003；河南新乡453003；

出版日期:2008-02-15 发布日期:2011-08-24

Column Chromatography Characteristics of Cordyceps sinensis Polysaccharides

WU Zhong-Wei, LIU Ming-Jiu, DOU Yan-Ping, ZHAO Xian-Fang

1.Department of Bioengineering, Henan Institute of Science and Technology, Xinxiang 453003, China;2.Department of Agriculture, Henan Insititute of Science and Technology, Xinxiang 453003, China

Online:2008-02-15 Published:2011-08-24

摘要/Abstract

摘要： 分级醇沉、分步醇沉和DEAE-32纤维素柱层析法研究了虫草多糖的分子量和多糖组分分布。分级醇沉实验表明,各醇沉浓度对应的得率都随醇浓度的升高而呈增加趋势,多糖含量变化起伏较大,醇浓度为30%和70%时所得多糖含量较高,虫草胞内和胞外多糖分子量分布都很不均匀;分步醇沉测定了各部分多糖所占的比例。DEAE-32纤维素柱层析实验结果表明:冬虫夏草的胞外多糖和胞内多糖具有分子量相似的中性多糖峰,并且它们的含量较为接近;二者的酸性多糖组分也具有相似的规律。

关键词: 虫草多糖, 醇沉, DEAE-32纤维素, 柱层析

Abstract: The molecular weight and components distribution of Cordyceps sinensis polysaccharides were studied by ethanol precipitation(step and stepwise) and DEAE-32 cellulose column chromatography respectively. The experimental results of stepwise ethanol precipitation showed that the yeild represents the increasing trend with increase of the ethanol concentration, their polysaccharides contents have significant changes, and at the ethanol concentration of 30% and 70%, polysaccharides contents are up to maximum. Molecular weights of endopolysaccharides and exopolysaccharides are pockety. During step ethanol precipitation the contents of the different components of Cordyceps sinensis polysaccharides were assayed. The results showed that endopolysaccharides and exopolysaccharides have the similar molecular weight and content;and also their acidic polysaccharides have the similar regulation by DEAE-32 cellulose column chromatography.

Key words: Cordyceps sinensis polysaccharides, ethanol precipitation, DEAE-32 cellulose, column chromatography

武忠伟, 刘明久, 窦艳萍, 赵现方.
虫草多糖醇沉和DEAE-32纤维素柱层析特性研究[J]. 食品科学, 2008, 29(2): 86-90.

WU Zhong-Wei, LIU Ming-Jiu, DOU Yan-Ping, ZHAO Xian-Fang. Column Chromatography Characteristics of Cordyceps sinensis Polysaccharides[J]. FOOD SCIENCE, 2008, 29(2): 86-90.

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