食品科学 ›› 2008, Vol. 29 ›› Issue (3): 152-157.

• 基础研究 • 上一篇    下一篇

中性蛋白酶和碱性蛋白酶对玉米蛋白水解作用的研究

 何莉萍, 刘良忠   

  1. 武汉工业学院食品科学与工程学院; 武汉工业学院食品科学与工程学院 湖北武汉430023; 湖北武汉430023;
  • 出版日期:2008-03-15 发布日期:2011-08-24

Study on Hydrolysis Effects of Neutral Protease and Alkaline Protease on Corn Protein

 HE  Li-Ping, LIU  Liang-Zhong   

  1. College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430023, China
  • Online:2008-03-15 Published:2011-08-24

摘要: 本实验研究了中性蛋白酶和碱性蛋白酶分别作用和共同作用对玉米蛋白粉中玉米蛋白的水解效果。结果表明,中性蛋白酶水解玉米蛋白的较佳水解条件是:酶浓度3544U/g、pH7.0、温度55℃、水解时间(t)为2h;碱性蛋白酶水解玉米蛋白粉的较佳水解条件是:酶浓度840U/g、pH8.0、温度55℃、水解时间2h。采用中性蛋白酶和碱性蛋白酶复合水解玉米蛋白比单一酶水解的效果好,水解液的水解度达到20%以上。

关键词: 玉米蛋白, 酶, 水解

Abstract: Hydrolysis effect of alkaline protease and neutral protease by single and compound on corn protein was studied. The optimum hydrolyzing conditions for neutral protease have been determined: [E] 3544 U/g, pH 7.0, temperature 55 ℃ and time(t) 2 h; The optimum hydrolyzing conditions for alkaline protease: [E] 840 U/g, pH 8.0, temperature 55 ℃ and time 2 h. In addition, two protease compound hydrolyzing corn protein is better than the single, and its DH can achieve above 20%.

Key words: corn protein power, protease, degree of hydrolysis