食品科学 ›› 2008, Vol. 29 ›› Issue (3): 340-345.

• 生物工程 • 上一篇    下一篇

青腐乳生产菌总状毛霉的鉴定及致病性研究

 吴彩梅, 王静, 孙伟伟, 曹维强, 吴秀群   

  1. 四川农业大学动物营养研究所; 中国农业科学院农业质量标准与检测技术研究所; 滨州学院自动化系; 惠州出入境检验检疫局; 四川农业大学动物营养研究所 四川雅安625014 中国农业科学院农业质量标准与检测技术研究所; 北京100081; 山东滨州256600; 广东惠州516001; 四川雅安625014;
  • 出版日期:2008-03-15 发布日期:2011-08-24

Identification and Pathogenicity Study on Gray Sufu Processing Mucor M.racemosu with Mice Experiment

 WU  Cai-Mei, WANG  Jing, SUN  Wei-Wei, CAO  Wei-Qiang, WU  Xiu-Qun   

  1. 1.Animal Nutrition Institute, Sichuan Agricultural University, Ya’an 625014, China; 2.Institute of Quality Standards and Testing Technology for Agri-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, China;3.Automation Department, Binzhou University, Binzhou 256600, China; 4.Huizhou Entry-Exit Inspection and Quarantine Bureau, Huizhou 516001, China
  • Online:2008-03-15 Published:2011-08-24

摘要: 本研究对腐乳生产厂家提供的生产菌进行了鉴定,确定为总状毛霉。并对该菌进行了致病性研究。将不同浓度的总状毛霉孢子经小鼠腹腔注射,观察30d内动物出现的中毒症状、死亡和体重变化,对实验中死亡和定期处死的小鼠解剖,测定脑、肝、脾、肾、肺、心等组织中的生存菌数,同时作病理组织学检查。结果表明,该菌对小鼠造成的主要损害为肝脏坏死性病变,随染孢子量增加,小鼠各脏器中检出的活菌数增多,其中5×106(个/只)及其以上剂量组所有脏器可同时检出活孢子,且有正剂量反应关系。但致病性与染孢子量不呈正剂量反应关系。

关键词: 青腐乳, 总状毛霉, 鉴定, 致病性

Abstract:  At first, gray sufu producing mucor supplied by sufu manufacturer in Harbin was identified in the study. The producing mucor was identified for M.racemosu in the end. The second, the pathogenicity of M.racemosu was also studied. The pathogenicity experiments of M.racemosu were completed by injecting the suspension of different levels of spores in celiac of mice, observing poisoning symptom, death condition and the weight changes of mice within 30 d, meanwhile, the mice which had died and was executed were anatomized. The viscera including brain, liver, spleen, kidney, lung, heart were collected to examinate pathological changes by HE dye and the counts of survival M.racemosus. The result indicated that the mucor can result in heart, brain, liver, kidney, spleen and lung making pathological changes, especially liver. M.racemosu can mainly result in the putrescence focus of liver of mice. The counts of survival M.racemosus in viscera would increase with increase of injected the counts of M.racemosus spore. In the 5×106 and 5×107 dosage groups , living spore can be observed in all viscera at the same time. And there was positive relationship between living spore and dosage. But, there was not positive relationship between pathogenicity and dosage.

Key words: gray sufu, M.racemosus, identification, pathogenicity