食品科学 ›› 2008, Vol. 29 ›› Issue (8): 467-470.

• 生物工程 • 上一篇    下一篇

利用啤酒麦糟进行L-乳酸生产的研究

邵荣, 余晓红, 刘珊珊   

  1. 盐城工学院化学与生物工程学院
  • 出版日期:2008-08-15 发布日期:2011-08-26

Study on of Preparation of L-Lactic Acid with Brewer’s Spent Grains

 SHAO  Rong, YU  Xiao-Hong, LIU  Shan-Shan   

  1. College of Chemistery and Biological Engineering,Yancheng Institute of Technology,Yancheng 224003,China
  • Online:2008-08-15 Published:2011-08-26

摘要: 将湿啤酒糟烘干至含水量为10%左右,再进行机械粉碎,过20~40目筛子筛分后,采用0.80%硫酸,固液比l:11,浸泡原料10h后,于121℃下预水解90min。预水解物pH值用2.0%的氢氧化钠调至4.0左右后,添加0.20%(W/V)左右的纤维素酶,于50℃酶解5h,得到还原糖含量为8.82%的啤酒糟酶解液。将此酶解液pH调节到6.0左右,于55℃采用分批补料的发酵方式进行发酵控制,添加5.0%的碳酸钙,可得到乳酸产量为80.75g/L的发酵液。

关键词: 啤酒糟, 酶水解, 乳酸发酵

Abstract: Wet brewer’s spent grain was dried to the water content around 10%,and then smashed with the undersized machine. After 20 to 40 mesh screening,grains were soaked with 0.80% H2SO4 for 10 h at the ratio of solid to liguid 1:11,and then were prehydrolyzed for 90 min at 121 ℃. The pre-hydrolysates were hydrolyzed with 0.20% cellulase(W/V) for 5 h at 50 ℃ after adjusting pH to around 4.0 with 2.0 % NaOH,and the hydrolysate with 8.82 % reducing sugar content was obtain. After adjusting pH to around 6.0,the hydrolysate added with 5.0 % CaCO3 was fermented at 55 ℃ by batch feeding,and the yield of L-lactic acid in the fermentation broth is 80.75 g/L.

Key words: brewer&rsquo, s spent grains; enzymic hydrolysis; lactic acid fermentation